Mouse Non Metastatic Cells 1, Protein NM23A Expressed In (NME1) RTU ELISA Kit | NME1 rtu elisa kit

Mouse Non Metastatic Cells 1, Protein NM23A Expressed In (NME1) ELISA Kit

Cat. Num. AAA4503583

• Gene Names: Nme1; NM23A; NDPK-A; NM23-M1; AL024257
• Reactivity: Mouse
• Synonyms: Non Metastatic Cells 1; Protein NM23A Expressed In (NME1); Mouse Non Metastatic Cells 1; Protein NM23A Expressed In (NME1) ELISA Kit; AWD; GAAD; NDPKA; NM23-H1; Nucleoside diphosphate kinase A; Granzyme A-activated DNase; Metastasis inhibition factor nm23; Tumor metastatic process-associated protein; NME1 rtu elisa kit

• Reactivity: Mouse
• Sequence Length: 152

• Assay Type: Sandwich
• Samples: Serum, Plasma or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.053ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NME1 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NME1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to NME1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NME1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of NME1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 37700232
• NCBI GeneID: 18102
• NCBI Accession #: NP_032730.1
• NCBI GenBank Nucleotide #: NM_008704.2
• UniProt Accession #: P15532
• Molecular Weight: 17,208 Da
• NCBI Official Full Name: nucleoside diphosphate kinase A
• NCBI Official Synonym Full Names: NME/NM23 nucleoside diphosphate kinase 1
• NCBI Official Symbol: Nme1
• NCBI Official Synonym Symbols: NM23A; NDPK-A; NM23-M1; AL024257
• NCBI Protein Information: nucleoside diphosphate kinase A
• UniProt Protein Name: Nucleoside diphosphate kinase A
• Protein Family: Nucleoside diphosphate kinase
• UniProt Gene Name: Nme1
• UniProt Synonym Gene Names: Nm23; NDK A; NDP kinase A

Uniprot Description

Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Possesses nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase, histidine protein kinase and 3'-5' exonuclease activities. Involved in cell proliferation, differentiation and development, signal transduction, G protein-coupled receptor endocytosis, and gene expression. Required for neural development including neural patterning and cell fate determination. During GZMA-mediated cell death, works in concert with TREX1. NME1 nicks one strand of DNA and TREX1 removes bases from the free 3' end to enhance DNA damage and prevent DNA end reannealing and rapid repair ().