Western Blot (WB)
(Western blot analysis of DGK theta expression in K562 (A), MCF7 (B), mouse kidney (C) whole cell lysates.)
Rabbit anti-Human, Mouse DGK theta Polyclonal Antibody | anti-DGKQ antibody
Anti-DGK theta Antibody
IHC: 1:50-1:200
Western Blot (WB)
(Western blot analysis of DGK theta expression in K562 (A), MCF7 (B), mouse kidney (C) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of DGK theta expression in K562 (A), MCF7 (B), mouse kidney (C) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of DGK theta staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of DGK theta staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene contains three cysteine-rich domains, a proline-rich region, and a pleckstrin homology domain with an overlapping Ras-associating domain. It is localized in the speckle domains of the nucleus, and mediates the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction. [provided by RefSeq, Jul 2008]
Uniprot Description
DGKQ: Phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA). May regulate the activity of protein kinase C by controlling the balance between these two signaling lipids. Activated in the nucleus in response to alpha-thrombin and nerve growth factor. May be involved in cAMP- induced activation of NR5A1 and subsequent steroidogenic gene transcription by delivering PA as ligand for NR5A1. Acts synergistically with NR5A1 on CYP17 transcriptional activity. Belongs to the eukaryotic diacylglycerol kinase family.
Protein type: Kinase, lipid; Motility/polarity/chemotaxis; Lipid Metabolism - glycerolipid; Lipid Metabolism - glycerophospholipid; EC 2.7.1.107
Chromosomal Location of Human Ortholog: 4p16.3
Cellular Component: cytoskeleton; cytoplasm; plasma membrane; nuclear speck; cytosol; nucleus
Molecular Function: protein binding; phospholipase binding; transcription activator binding; metal ion binding; kinase binding; diacylglycerol kinase activity; ATP binding; NAD+ kinase activity
Biological Process: regulation of transcription from RNA polymerase II promoter; platelet activation; G-protein coupled receptor protein signaling pathway; cAMP-mediated signaling; protein kinase C activation; blood coagulation; phosphorylation; response to ATP
Research Articles on DGKQ
Similar Products
Product Notes
The DGKQ dgkq (Catalog #AAA8306460) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-DGK theta Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's DGK theta can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the DGKQ dgkq for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DGK theta, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
