Human CRLF2 (Cardiotrophin-like cytokine factor 2) ELISA Kit | CRLF2 elisa kit

Human CRLF2 (Cardiotrophin-like cytokine factor 2) ELISA Kit

Cat. Num. AAA7612299

• Gene Names: CRLF2; CRL2; TSLPR; CRLF2Y
• Reactivity: Human
• Synonyms: CRLF2 (Cardiotrophin-like cytokine factor 2); Human CRLF2 (Cardiotrophin-like cytokine factor 2) ELISA Kit; Cytokine receptor-like factor 2; Cytokine receptor-like 2; IL-XR; Thymic stromal lymphopoietin protein receptor; TSLP receptor; CRLF2; CRLF2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of CRLF2. No significant cross-reactivity or interference between CRLF2 and analogues was observed.

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 31.25-2000pg/ml
• Sensitivity: 18.75pg/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 2-8 degree C for 6 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CRLF2 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 589269166
• NCBI GeneID: 64109
• NCBI Accession #: NP_001012288.2
• NCBI GenBank Nucleotide #: NM_001012288.2
• UniProt Accession #: Q9HC73; Q5G7M1; Q9H5R3; J3QKD1
• Molecular Weight: 29,360 Da
• NCBI Official Full Name: cytokine receptor-like factor 2 isoform 2
• NCBI Official Synonym Full Names: cytokine receptor-like factor 2
• NCBI Official Symbol: CRLF2
• NCBI Official Synonym Symbols: CRL2; TSLPR; CRLF2Y
• NCBI Protein Information: cytokine receptor-like factor 2; IL-XR; TSLP receptor; cytokine receptor CRL2 precusor; thymic stromal lymphopoietin protein receptor; thymic stromal-derived lymphopoietin receptor
• UniProt Protein Name: Cytokine receptor-like factor 2
• Protein Family: Cytokine receptor-like factor
• UniProt Gene Name: CRLF2
• UniProt Synonym Gene Names: CRL2; ILXR; TSLPR; TSLP receptor
• UniProt Entry Name: CRLF2_HUMAN

NCBI Description

This gene encodes a member of the type I cytokine receptor family. The encoded protein is a receptor for thymic stromal lymphopoietin (TSLP). Together with the interleukin 7 receptor (IL7R), the encoded protein and TSLP activate STAT3, STAT5, and JAK2 pathways, which control processes such as cell proliferation and development of the hematopoietic system. Rearrangement of this gene with immunoglobulin heavy chain gene (IGH) on chromosome 14, or with P2Y purinoceptor 8 gene (P2RY8) on the same X or Y chromosomes is associated with B-progenitor acute lymphoblastic leukemia (ALL) and Down syndrome ALL. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2014]

Uniprot Description

CRLF2: Receptor for thymic stromal lymphopoietin (TSLP). Forms a functional complex with TSLP and IL7R which is capable of stimulating cell proliferation through activation of STAT3 and STAT5. Also activates JAK2. Implicated in the development of the hematopoietic system. Belongs to the type I cytokine receptor family. Type 5 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: Xp22.3; Yp11.3

Cellular Component: extracellular region; plasma membrane; integral to membrane