Western Blot (WB)
(Anti-NPRL3 Antibody (N-Term) at 1:2000 dilution + HT-1080 whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 64 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Human NPRL3 Polyclonal Antibody | anti-NPRL3 antibody
NPRL3 Antibody (N-Term)
Gene Names
NPRL3; MARE; NPR3; HS-40; RMD11; CGTHBA; C16orf35
Reactivity
Human
Applications
Western Blot, ELISA
Synonyms
NPRL3; Polyclonal Antibody; NPRL3 Antibody (N-Term); Purified Rabbit Polyclonal Antibody (Pab); Nitrogen permease regulator 3-like protein; -14 gene protein; Alpha-globin regulatory element-containing gene protein; Protein CGTHBA; C16orf35; CGTHBA; MARE; anti-NPRL3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Ig
Specificity
This NPRL3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 86-117 amino acids from human NPRL3.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Length
569
Applicable Applications for anti-NPRL3 antibody
Western Blot (WB), ELISA (E)
Application Notes
WB ~~ 1:2000
Antigen Source
Human
Function
As a component of the GATOR1 complex functions as an inhibitor of the amino acid-sensing branch of the TORC1 pathway. The GATOR1 complex strongly increases GTP hydrolysis by RRAGA and RRAGB within RRAGC-containing heterodimers, thereby deactivating RRAGs, releasing mTORC1 from lysosomal surface and inhibiting mTORC1 signaling. The GATOR1 complex is negatively regulated by GATOR2 the other GATOR subcomplex in this amino acid-sensing branch of the TORC1 pathway.
Cellular Location
Lysosome membrane. Note=Localization to lysosomes is amino acid-independent.
Tissue Location
Widely expressed. Expressed in the frontal lobe cortex as well as in the temporal, parietal, and occipital lobes (PubMed:27173016, PubMed:26505888)
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(Anti-NPRL3 Antibody (N-Term) at 1:2000 dilution + HT-1080 whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 64 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Anti-NPRL3 Antibody (N-Term) at 1:2000 dilution + HT-1080 whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 64 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-NPRL3 antibody
As a component of the GATOR1 complex, inhibitor of the amino acid-sensing branch of the TORC1 pathway. The GATOR1 complex strongly increases GTP hydrolysis by RRAGA and RRAGB within RRAGC- containing heterodimers, thereby deactivating RRAGs, releasing mTORC1 from lysosomal surface and inhibiting mTORC1 signaling.
Product Categories/Family for anti-NPRL3 antibody
References
Vyas P.,et al.Genomics 29:679-689(1995).
De Gobbi M.,et al.Science 312:1215-1217(2006).
Martin J.,et al.Nature 432:988-994(2004).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Neklesa T.K.,et al.PLoS Genet. 5:E1000515-E1000515(2009).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
63605
NCBI Official Full Name
nitrogen permease regulator 3-like protein isoform 1
NCBI Official Synonym Full Names
NPR3-like, GATOR1 complex subunit
NCBI Official Symbol
NPRL3
NCBI Official Synonym Symbols
MARE; NPR3; HS-40; RMD11; CGTHBA; C16orf35
NCBI Protein Information
nitrogen permease regulator 3-like protein
UniProt Protein Name
Nitrogen permease regulator 3-like protein
UniProt Gene Name
NPRL3
UniProt Synonym Gene Names
C16orf35; CGTHBA; MARE
UniProt Entry Name
NPRL3_HUMAN
NCBI Description
The function of the encoded protein is not known. [provided by RefSeq, Aug 2011]
Uniprot Description
NPRL3: The function of the encoded protein is not known. [provided by RefSeq, Aug 2011]
Protein type: Unknown function
Chromosomal Location of Human Ortholog: 16p13.3
Molecular Function: GTPase activator activity
Biological Process: cardiac muscle development; negative regulation of TOR signaling pathway; cellular response to amino acid starvation; palate development; positive regulation of GTPase activity
Research Articles on NPRL3
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Product Notes
The NPRL3 nprl3 (Catalog #AAA9215982) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NPRL3 Antibody (N-Term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NPRL3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (E). WB ~~ 1:2000. Researchers should empirically determine the suitability of the NPRL3 nprl3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NPRL3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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