Rabbit DEPDC5 Antibody | anti-DEPDC5 antibody

DEPDC5 Antibody

Cat. Num. AAA8502990

• Gene Names: DEPDC5; DEP.5; FFEVF; FFEVF1
• Reactivity: Reacts with: Human; Predicted: Rat, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Synonyms: DEPDC5; DEPDC5 Antibody; anti-DEPDC5 antibody

• Host: Rabbit
• Reactivity: Reacts with: Human; Predicted: Rat, Mouse
• Form/Format: Rabbit anti-DEPDC5 antibody purified by protein A column containing 0.02% NaN₃
• Sequence Length: 2397

• Applicable Applications for anti-DEPDC5 antibody: ELISA, Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: ELISA titer: 1:20,000-1:80,000; Western blot analysis: 1:200-1:1,000; Immunohistochemistry: 1:100-1:500; Optimal dilutions/concentrations should be determined by the end use
• Host Note: Rabbit polyclonal DEPDC5 ⁽¹⁾ antibody was raised against a recombinate human DEPDC5 protein 129aa-332aa (BC057797).
• Preparation and Storage: Already reconstituted in 200 ul sterile H₂O. ; The antibody is stable for at least one year when stored at -20°C after reconstitution. ; Avoid repeated freeze/defrost cycles.

Western Blot (WB)

Immunohistochemistry (IHC)

NCBI and Uniprot Product Information

• NCBI GI #: 34785517
• NCBI GeneID: 9681
• NCBI Official Full Name: Homo sapiens DEP domain containing 5, mRNA
• NCBI Official Synonym Full Names: DEP domain containing 5
• NCBI Official Symbol: DEPDC5
• NCBI Official Synonym Symbols: DEP.5; FFEVF; FFEVF1
• NCBI Protein Information: GATOR complex protein DEPDC5
• UniProt Protein Name: GATOR complex protein DEPDC5
• Protein Family: DEP domain-containing protein
• UniProt Gene Name: DEPDC5

NCBI Description

This gene encodes a member of the IML1 family of proteins involved in G-protein signaling pathways. The mechanistic target of rapamycin complex 1 (mTORC1) pathway regulates cell growth by sensing the availability of nutrients. The protein encoded by this gene is a component of the GATOR1 (GAP activity toward Rags) complex which inhibits the amino acid-sensing branch of the mTORC1 pathway. Mutations in this gene are associated with autosomal dominant familial focal epilepsy with variable foci. A single nucleotide polymorphism in an intron of this gene has been associated with an increased risk of hepatocellular carcinoma in individuals with chronic hepatitis C virus infection. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]

Uniprot Description

As a component of the GATOR1 complex functions as an inhibitor of the amino acid-sensing branch of the TORC1 pathway. The GATOR1 complex strongly increases GTP hydrolysis by RRAGA and RRAGB within RRAGC-containing heterodimers, thereby deactivating RRAGs, releasing mTORC1 from lysosomal surface and inhibiting mTORC1 signaling. The GATOR1 complex is negatively regulated by GATOR2 the other GATOR subcomplex in this amino acid-sensing branch of the TORC1 pathway.