NGFR active protein

NGFR Protein, Mouse, Recombinant (hFc Tag)

Cat. Num. AAA8123065

• Gene Names: Ngfr; p75; LNGFR; p75NTR; p75NGFR; Tnfrsf16
• Purity: >90% as determined by SDS-PAGE
• Synonyms: NGFR; NGFR Protein; Mouse; Recombinant (hFc Tag); Mouse NGFR/P75 Protein (Fc Tag); LNGFR Protein; p75 Protein; p75NGFR Protein; p75NTR Protein; RP23-67E18.6 Protein; Tnfrsf16 Protein; nerve growth factor receptor (TNFR superfamily; member 16); NGFR active protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Asn243

• Species: Mouse
• Activity: Measured by its ability to inhibit NGF-dependent proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.5-3ug/mL in the presence of 2ng/mL Recombinant mouse NGF.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Lys 22
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the extracellular domain of mouse NGFR (Q9Z0W1)(Met 1-Asn 243) was fused with the Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for NGFR active protein

Background: Nerve growth factor receptors (NGFRs) belong to a large growth factor receptor family. NGFR includes two types of receptors: high-affinity nerve growth factor receptor and low-affinity nerve growth factor receptor. The high-affinity nerve growth factor receptor is also referred to as the Trk family whose members are bound by some neurotrophins with high affinity. Nerve growth factor binds with TrkA after being released from target cells, the NGF/TrkA complex is subsequently trafficked back to the cell body. The Low-affinity nerve growth factor receptor also named p75 which binds with all kinds of neurotrophins with low affinity. All four kinds of neurotrophins, including Nerve growth factor, Brain-derived neurotrophic factor, Neurotrophin-3, and Neurotrophin-4 bind to the p75. Studies have proved that NGFR acts as a molecular signal switch that determines cell death or survival by three steps. First, pro-nerve growth factor (prNGF) triggers cell apoptosis by its high-affinity binding to p75NTR, while NGF induces neuronal survival with low-affinity binding. Second, p75NTR mediates cell death by combining with co-receptor Sortilin, whereas it promotes neuronal survival through combination with proNGF. Third, the release of the intracellular domain chopper or cleavage short p75 NTR can independently initiate neuronal apoptosis.

Product Categories/Family for NGFR active protein

Death Receptor Signaling

References

Chen LW, et al. (2008) The proNGF-p75NTR-sortilin signalling complex as new target for the therapeutic treatment of Parkinson's disease. CNS Neurol Disord Drug Targets. 7(6): 512-23.Deponti D, et al. (2009) The low-affinity receptor for neurotrophins p75NTR plays a key role for satellite cell function in muscle repair acting via RhoA. Mol Biol Cell.20(16): 3620-7.Ken-ichiro K, et al. (2004) Necdin-related MAGE proteins differentially interact with the E2F1 transcription factor and the p75 neurotrophin receptor. J Biol Chem. 279 (3): 1703-12.

NCBI and Uniprot Product Information

• NCBI GI #: 21264104
• NCBI GeneID: 18053
• UniProt Accession #: Q9Z0W1
• Molecular Weight: 50kDa
• NCBI Official Full Name: Tumor necrosis factor receptor superfamily member 16
• NCBI Official Synonym Full Names: nerve growth factor receptor (TNFR superfamily, member 16)
• NCBI Official Symbol: Ngfr
• NCBI Official Synonym Symbols: p75; LNGFR; p75NTR; p75NGFR; Tnfrsf16
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 16
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 16
• Protein Family: Tumor necrosis factor receptor superfamily
• UniProt Gene Name: Ngfr
• UniProt Synonym Gene Names: Tnfrsf16; NGF receptor

Uniprot Description

Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells (). Plays a role in the regulation of the translocation of GLUT4 to the cell surface in adipocytes and skeletal muscle cells in response to insulin, probably by regulating RAB31 activity, and thereby contributes to the regulation of insulin-dependent glucose uptake. Binds to rabies virus glycoprotein Gs. Necessary for the circadian oscillation of the clock genes ARNTL/BMAL1, PER1, PER2 and NR1D1 in the suprachiasmatic nucleus (SCN) of the brain and in liver and of the genes involved in glucose and lipid metabolism in the liver.