Rabbit anti-Human TWEAK Polyclonal Antibody | anti-TWEAK antibody

TWEAK (P73) Polyclonal Antibody

Cat. Num. AAA3002640

• Gene Names: TNFSF12; APO3L; DR3LG; TWEAK; TNLG4A
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: TWEAK; Polyclonal Antibody; TWEAK (P73) Polyclonal Antibody; Tumor necrosis factor ligand superfamily member 12; APO3 ligand; TNF-related weak inducer of apoptosis; TNFSF12; APO3L; DR3LG; UNQ181/PRO207; anti-TWEAK antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: TWEAK (P73) polyclonal antibody detects endogenous levels of TWEAK protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 249

• Applicable Applications for anti-TWEAK antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 51-100 of Human TWEAK.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of TWEAK (P73) polyclonal antibody at 1:500 dilutionLane1:A549 whole cell lysate (40ug) Lane2:U-87MG whole cell lysate (40ug) Lane3:HEK293T whole cell lysate (40ug) Lane4:A2780 whole cell lysate (40ug) Lane5:SP2/0 whole cell lysate (40ug) Lane6:C6 whole cell lysate (40ug) Lane7:PC12 whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of TWEAK (P73) pAb in paraffin-embedded human colon carcinoma tissue at 1:50.showing secreted and cell membrane staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Related Product Information for anti-TWEAK antibody

TWEAK is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein is a ligand for the FN14/TWEAKR receptor. It is widely expressed, has high constitutive expression in heart placenta, lung, muscle, kidney, pancreas, and HUVEC. This cytokine has overlapping signaling functions with TNF, but displays a much wider tissue distribution. TWEAK can induce apoptosis via multiple pathways of cell death in a cell type specific manner. This cytokine is also found to promote proliferation and migration of endothelial cells, and thus acts as a regulator of angiogenesis.

NCBI and Uniprot Product Information

• NCBI GI #: 4507597
• NCBI GeneID: 8742
• NCBI Accession #: NP_003800.1
• NCBI GenBank Nucleotide #: NP_003800.1
• UniProt Accession #: O43508
• Molecular Weight: ~ 27kDa
• NCBI Official Full Name: tumor necrosis factor ligand superfamily member 12 proprotein
• NCBI Official Synonym Full Names: TNF superfamily member 12
• NCBI Official Symbol: TNFSF12
• NCBI Official Synonym Symbols: APO3L; DR3LG; TWEAK; TNLG4A
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 12
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 12
• UniProt Gene Name: TNFSF12
• UniProt Synonym Gene Names: APO3L; DR3LG; TWEAK
• UniProt Entry Name: TNF12_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein is a ligand for the FN14/TWEAKR receptor. This cytokine has overlapping signaling functions with TNF, but displays a much wider tissue distribution. This cytokine, which exists in both membrane-bound and secreted forms, can induce apoptosis via multiple pathways of cell death in a cell type-specific manner. This cytokine is also found to promote proliferation and migration of endothelial cells, and thus acts as a regulator of angiogenesis. Alternative splicing results in multiple transcript variants. Some transcripts skip the last exon of this gene and continue into the second exon of the neighboring TNFSF13 gene; such read-through transcripts are contained in GeneID 407977, TNFSF12-TNFSF13. [provided by RefSeq, Oct 2010]

Uniprot Description

TNFSF12: Binds to FN14 and possibly also to TNRFSF12/APO3. Weak inducer of apoptosis in some cell types. Mediates NF-kappa-B activation. Promotes angiogenesis and the proliferation of endothelial cells. Also involved in induction of inflammatory cytokines. Homotrimer (Potential). Interacts with the angiogenic factor AGGF1/VG5Q. Highly expressed in adult heart, pancreas, skeletal muscle, brain, colon, small intestine, lung, ovary, prostate, spleen, lymph node, appendix and peripheral blood lymphocytes. Low expression in kidney, testis, liver, placenta, thymus and bone marrow. Also detected in fetal kidney, liver, lung and brain. Belongs to the tumor necrosis factor family.

Protein type: Motility/polarity/chemotaxis; Apoptosis; Cytokine; Membrane protein, integral

Chromosomal Location of Human Ortholog: 17p13

Cellular Component: extracellular space; integral to plasma membrane; perinuclear region of cytoplasm

Molecular Function: protein binding; cytokine activity; tumor necrosis factor receptor binding; receptor binding

Biological Process: positive regulation of angiogenesis; apoptosis; positive regulation of protein catabolic process; endothelial cell migration; immune response; positive regulation of endothelial cell proliferation; angiogenesis; cell differentiation; signal transduction