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Western Blot (WB) (Western blotAll lanes: DDHD2 antibody at 10ug/mlLane 1: K562 whole cell lysateLane 2: U251 whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 82,38 kDaObserved band size: 81 kDa)

Rabbit anti-Human DDHD2 Polyclonal Antibody | anti-DDHD2 antibody

DDHD2 Antibody

Gene Names
DDHD2; SPG54; SAMWD1; iPLA(1)gamma
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity Purified
Synonyms
DDHD2; Polyclonal Antibody; DDHD2 Antibody; Phospholipase DDHD2 (EC:3.1.1.-); KIAA0725; SAMWD1; DDHD domain-containing protein 2; KIAA0725p; SAM; WWE and DDHD domain-containing protein 1; anti-DDHD2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purified
Form/Format
Liquid
Sequence Length
711
Applicable Applications for anti-DDHD2 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Recommended dilution: WB:1:500-2000, IHC:1:20-1:200
Immunogen
Recombinant human Phospholipase DDHD2 protein
Conjugation
Non-conjugated
Storage Buffer
Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-87679
Preparation and Storage
Shipped at 4 degree C. Upon delivery, aliquot and store at -20 degree C or -80 degree C.

Western Blot (WB)

(Western blotAll lanes: DDHD2 antibody at 10ug/mlLane 1: K562 whole cell lysateLane 2: U251 whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 82,38 kDaObserved band size: 81 kDa)

Western Blot (WB) (Western blotAll lanes: DDHD2 antibody at 10ug/mlLane 1: K562 whole cell lysateLane 2: U251 whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 82,38 kDaObserved band size: 81 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human small intestine using MBS7004676 at dilution of 1:100)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human small intestine using MBS7004676 at dilution of 1:100)
Related Product Information for anti-DDHD2 antibody
Phospholipase that hydrolyzes preferentially phosphatidic acid, including 1,2-dioleoyl-sn-phosphatidic acid, and phosphatidylethanolamine. Specifically binds to phosphatidylinositol 3-phosphate (PI(3)P), phosphatidylinositol 4-phosphate (PI(4)P), phosphatidylinositol 5-phosphate (PI(5)P) and possibly phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2). May be involved in the maintenance of the endoplasmic reticulum and/or Golgi structures. May regulate the transport between Golgi apparatus and plasma membrane.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
37,682 Da
NCBI Official Full Name
phospholipase DDHD2 isoform 1
NCBI Official Synonym Full Names
DDHD domain containing 2
NCBI Official Symbol
DDHD2
NCBI Official Synonym Symbols
SPG54; SAMWD1; iPLA(1)gamma
NCBI Protein Information
phospholipase DDHD2
UniProt Protein Name
Phospholipase DDHD2
Protein Family
UniProt Gene Name
DDHD2
UniProt Synonym Gene Names
KIAA0725; SAMWD1
UniProt Entry Name
DDHD2_HUMAN

NCBI Description

This gene encodes a phospholipase enzyme containing sterile-alpha-motif (SAM), WWE, and DDHD domains. This protein participates in membrane trafficking between the endoplastic reticulum and the Golgi body. Mutations in this gene can cause autosomal recessive spastic paraplegia 54. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]

Uniprot Description

DDHD2: Phospholipase that hydrolyzes preferentially phosphatidic acid and phosphatidylethanolamine. May be involved in the maintenance of the endoplasmic reticulum and/or Golgi structures. Belongs to the PA-PLA1 family.

Protein type: EC 3.1.1.-; Hydrolase

Chromosomal Location of Human Ortholog: 8p11.23

Cellular Component: cytoplasm; cytosol; ER-Golgi intermediate compartment; Golgi apparatus; microtubule organizing center

Molecular Function: hydrolase activity; metal ion binding

Biological Process: glycerophospholipid biosynthetic process; lipid catabolic process; phosphatidic acid biosynthetic process; phospholipid metabolic process

Disease: Spastic Paraplegia 54, Autosomal Recessive

Research Articles on DDHD2

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Product Notes

The DDHD2 ddhd2 (Catalog #AAA7004676) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DDHD2 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DDHD2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Recommended dilution: WB:1:500-2000, IHC:1:20-1:200. Researchers should empirically determine the suitability of the DDHD2 ddhd2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DDHD2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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