Western Blot (WB)
(Staining (1ug/ml) of A431 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Goat RXR beta Polyclonal Antibody | anti-RXRB antibody
Goat anti-RXR beta Antibody
Western Blot: Approx 55kDa band observed in lysates of cell line A431 (calculated MW of 56.9kDa according to NP_068811.1). In transfected HEK293 transiently expressing RXRB a band of approx. 60kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3ug/ml.
Western Blot (WB)
(Staining (1ug/ml) of A431 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Western Blot (WB)
(Staining (1ug/ml) of A431 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Western Blot (WB)
(HEK293 overexpressing RXRB (RC200516) and probed (mock transfection in first lane), tested by Origene.)
Western Blot (WB)
(HEK293 overexpressing RXRB (RC200516) and probed (mock transfection in first lane), tested by Origene.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the retinoid X receptor (RXR) family of nuclear receptors which are involved in mediating the effects of retinoic acid (RA). The encoded protein forms homodimers with the retinoic acid, thyroid hormone, and vitamin D receptors, increasing both DNA binding and transcriptional function on their respective response elements. This gene lies within the major histocompatibility complex (MHC) class II region on chromosome 6. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]
Uniprot Description
RXRB: Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. Specifically binds 9-cis retinoic acid (9C-RA). Homodimer. Heterodimer with a RAR molecule. Binds DNA preferentially as a RAR/RXR heterodimer. Expressed in a variety of tumor cell lines. Belongs to the nuclear hormone receptor family. NR2 subfamily.
Protein type: DNA-binding; Nuclear receptor
Chromosomal Location of Human Ortholog: 6p21.3
Cellular Component: nucleoplasm; nucleus
Molecular Function: ligand-dependent nuclear receptor activity; protein binding; protein heterodimerization activity; retinoic acid receptor binding; retinoid-X receptor activity; steroid hormone receptor activity; thyroid hormone receptor binding; thyroid hormone receptor coactivator activity; transcription coactivator activity; transcription factor activity; vitamin D receptor binding; zinc ion binding
Biological Process: positive regulation of transcription from RNA polymerase II promoter; regulation of myelination; retinoic acid receptor signaling pathway; steroid hormone mediated signaling; transcription initiation from RNA polymerase II promoter
Research Articles on RXRB
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Product Notes
The RXRB rxrb (Catalog #AAA420305) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-RXR beta Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's RXR beta can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 128000. Western Blot: Approx 55kDa band observed in lysates of cell line A431 (calculated MW of 56.9kDa according to NP_068811.1). In transfected HEK293 transiently expressing RXRB a band of approx. 60kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the RXRB rxrb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: CRDGMGDSGR DSRSP. It is sometimes possible for the material contained within the vial of "RXR beta, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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