Rabbit anti-Human, Mouse DCLRE1B Polyclonal Antibody | anti-DCLRE1B antibody

DCLRE1B, ID (DCLRE1B, SNM1B, 5' exonuclease Apollo, DNA cross-link repair 1B protein, SNM1 homolog B) (MaxLight 550)

Cat. Num. AAA6291119

• Gene Names: DCLRE1B; SNM1B; SNMIB; APOLLO
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DCLRE1B; Polyclonal Antibody; ID (DCLRE1B; SNM1B; 5' exonuclease Apollo; DNA cross-link repair 1B protein; SNM1 homolog B) (MaxLight 550); SNM1 homolog B; anti-DCLRE1B antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.

• Applicable Applications for anti-DCLRE1B antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-100; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: DCLRE1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 215-244 amino acids from the Central region of human DCLRE1B.
• Conjugate: MaxLight550
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Related Product Information for anti-DCLRE1B antibody

DNA interstrand cross-links prevent strand separation, thereby physically blocking transcription, replication, and segregation of DNA. DCLRE1B is one of several evolutionarily conserved genes involved in repair of interstrand cross-links (Dronkert et al., 2000 [PubMed 10848582]).

Product Categories/Family for anti-DCLRE1B antibody

Antibodies; DNA Replication; Repair

References

Anders, M., et al. Cell Cycle 8(11):1725-1732(2009). Liu, L., et al. Cell Cycle 8(4):628-638(2009). Freibaum, B.D., et al. J. Biol. Chem. 283(35):23671-23676(2008). Bae, J.B., et al. Oncogene 27(37):5045-5056(2008). Demuth, I., et al. DNA Repair (Amst.) 7(8):1192-1201(2008). Matsuoka, S., et al. Science 316(5828):1160-1166(2007). Lenain, C., et al. Curr. Biol. 16(13):1303-1310(2006). Freibaum, B.D., et al. J. Biol. Chem. 281(22):15033-15036(2006). Ishiai, M., et al. Mol. Cell. Biol. 24(24):10733-10741(2004). Demuth, I., et al. Oncogene 23(53):8611-8618(2004). Dronkert, M.L., et al. Mol. Cell. Biol. 20(13):4553-4561(2000).

NCBI and Uniprot Product Information

• NCBI GI #: 12383082
• NCBI GeneID: 64858
• NCBI Accession #: NP_073747.1
• NCBI GenBank Nucleotide #: NM_022836.3
• UniProt Accession #: Q9H816; Q9H9E5
• Molecular Weight: 60,002 Da
• NCBI Official Full Name: 5' exonuclease Apollo
• NCBI Official Synonym Full Names: DNA cross-link repair 1B
• NCBI Official Symbol: DCLRE1B
• NCBI Official Synonym Symbols: SNM1B; SNMIB; APOLLO
• NCBI Protein Information: 5' exonuclease Apollo; PSO2 homolog; SNM1 homolog B; DNA cross-link repair 1B (PSO2 homolog, S. cerevisiae)
• UniProt Protein Name: 5' exonuclease Apollo
• Protein Family: 5' exonuclease
• UniProt Gene Name: DCLRE1B
• UniProt Synonym Gene Names: SNM1B; SNMIB; hSNM1B
• UniProt Entry Name: DCR1B_HUMAN

NCBI Description

DNA interstrand cross-links prevent strand separation, thereby physically blocking transcription, replication, and segregation of DNA. DCLRE1B is one of several evolutionarily conserved genes involved in repair of interstrand cross-links (Dronkert et al., 2000 [PubMed 10848582]).[supplied by OMIM, Mar 2008]

Uniprot Description

DCLRE1B: 5'-3' exonuclease that plays a central role in telomere maintenance and protection during S-phase. Participates in the protection of telomeres against non-homologous end-joining (NHEJ)- mediated repair, thereby ensuring that telomeres do not fuse. Plays a key role in telomeric loop (T loop) formation by being recruited by TERF2 at the leading end telomeres and by processing leading-end telomeres immediately after their replication via its exonuclease activity: generates 3' single-stranded overhang at the leading end telomeres avoiding blunt leading-end telomeres that are vulnerable to end-joining reactions and expose the telomere end in a manner that activates the DNA repair pathways. Together with TERF2, required to protect telomeres from replicative damage during replication by controlling the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B) needed for telomere replication during fork passage and prevent aberrant telomere topology. Also involved in response to DNA damage: plays a role in response to DNA interstrand cross-links (ICLs) by facilitating double-strand break formation. In case of spindle stress, involved in prophase checkpoint. Defects in DCLRE1B may be a cause of Hoyeraal-Hreidarsson syndrome (HHS). HHS is a multisystem disorder affecting males and is characterized by aplastic anemia, immunodeficiency, microcephaly, cerebellar hypoplasia, and growth retardation. An aberrant splice variant designated Apollo- Delta has been found in a patient with Hoyeraal-Hreidarsson syndrome. Apollo-Delta hampers the proper replication of telomeres, leading to major telomeric dysfunction and cellular senescence, but maintains its DNA interstrand cross-link repair function in the whole genome. Belongs to the DNA repair metallo-beta-lactamase (DRMBL) family.

Protein type: DNA repair, damage; Hydrolase; EC 3.1.-.-

Chromosomal Location of Human Ortholog: 1p13.2

Cellular Component: centrosome; chromosome, telomeric region; cytoplasm; nucleus

Molecular Function: 5'-3' exonuclease activity; protein binding

Biological Process: telomeric loop formation; telomeric 3' overhang formation; DNA repair; telomere maintenance; cell cycle checkpoint; protection from non-homologous end joining at telomere