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SDS-Page (Figure 1. Human CD70 Protein, mFc-His Tag on SDS-PAGE under reducing condition.)

CD70 recombinant protein

Recombinant human CD70 protein with N-terminal mouse Fc and 6xHis tag

Gene Names
CD70; CD27L; CD27LG; TNFSF7
Applications
Western Blot
Purity
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Synonyms
CD70; Recombinant human CD70 protein with N-terminal mouse Fc and 6xHis tag; Human CD70 Protein; mFc-His Tag; CD70;CD27LG;TNFSF7;TNFSF7G;CD27L; CD70 recombinant protein
Ordering
For Research Use Only!
Host
HEK293 cells
Purity/Purification
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Form/Format
Lyophilized from sterile PBS, pH 7.4. Normally 5%-46% trehalose is added as protectants before lyophilization.
Sequence
mFc(Pro99-Lys330)+CD70(Gln39-Pro193)
Applicable Applications for CD70 recombinant protein
Western Blot (WB)
Source
Human
Conjugation
N-Mouse Fc and C-6xHis tag
Reconstitution
Reconstitute with deionized water
Preparation and Storage
Store at -80 degree C for 12 months (Avoid repeated freezing and thawing)
Shipping: Blue Ice

SDS-Page

(Figure 1. Human CD70 Protein, mFc-His Tag on SDS-PAGE under reducing condition.)

SDS-Page (Figure 1. Human CD70 Protein, mFc-His Tag on SDS-PAGE under reducing condition.)

ELISA (EIA)

(Figure 2. ELISA plate pre-coated by 2 ug/ml (100 ul/well) Human CD27, hFc tagged protein can bind Human CD70,mFc-His tagged protein in a linear range of 3.2-400 ng/ml.)

ELISA (EIA) (Figure 2. ELISA plate pre-coated by 2 ug/ml (100 ul/well) Human CD27, hFc tagged protein can bind Human CD70,mFc-His tagged protein in a linear range of 3.2-400 ng/ml.)
Related Product Information for CD70 recombinant protein
The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF27/CD27. It is a surface antigen on activated, but not on resting, T and B lymphocytes. It induces proliferation of costimulated T cells, enhances the generation of cytolytic T cells, and contributes to T cell activation. This cytokine is also reported to play a role in regulating B-cell activation, cytotoxic function of natural killer cells, and immunoglobulin sythesis.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
970
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
21,118 Da
NCBI Official Full Name
CD70 antigen
NCBI Official Synonym Full Names
CD70 molecule
NCBI Official Symbol
CD70
NCBI Official Synonym Symbols
CD27L; CD27LG; TNFSF7
NCBI Protein Information
CD70 antigen; CD27-L; CD27 ligand; Ki-24 antigen; surface antigen CD70; tumor necrosis factor ligand superfamily member 7; tumor necrosis factor (ligand) superfamily, member 7
UniProt Protein Name
CD70 antigen
Protein Family
UniProt Gene Name
CD70
UniProt Synonym Gene Names
CD27L; CD27LG; TNFSF7; CD27-L
UniProt Entry Name
CD70_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF27/CD27. It is a surface antigen on activated, but not on resting, T and B lymphocytes. It induces proliferation of costimulated T cells, enhances the generation of cytolytic T cells, and contributes to T cell activation. This cytokine is also reported to play a role in regulating B-cell activation, cytotoxic function of natural killer cells, and immunoglobulin sythesis. [provided by RefSeq, Jul 2008]

Uniprot Description

CD70: Cytokine that binds to CD27. Plays a role in T-cell activation. Induces the proliferation of costimulated T-cells and enhances the generation of cytolytic T-cells. Belongs to the tumor necrosis factor family.

Protein type: Cytokine; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19p13

Cellular Component: extracellular space; integral to plasma membrane; plasma membrane

Molecular Function: protein binding; protease binding; cytokine activity; tumor necrosis factor receptor binding; receptor binding

Biological Process: cell proliferation; cell-cell signaling; immune response; signal transduction

Research Articles on CD70

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Product Notes

The CD70 cd70 (Catalog #AAA188096) is a Recombinant Protein produced from HEK293 cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD70 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the CD70 cd70 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: mFc(Pro99- Lys330)+CD 70(Gln39-P ro193). It is sometimes possible for the material contained within the vial of "CD70, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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