Western Blot (WB)
(Western blot analysis of DCLRE1B expression in HOS (A) whole cell lysates.)
Rabbit anti-Human DCLRE1B Polyclonal Antibody | anti-DCLRE1B antibody
Anti-DCLRE1B Antibody
Western Blot (WB)
(Western blot analysis of DCLRE1B expression in HOS (A) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of DCLRE1B expression in HOS (A) whole cell lysates.)
NCBI and Uniprot Product Information
NCBI Description
DNA interstrand cross-links prevent strand separation, thereby physically blocking transcription, replication, and segregation of DNA. DCLRE1B is one of several evolutionarily conserved genes involved in repair of interstrand cross-links (Dronkert et al., 2000 [PubMed 10848582]).[supplied by OMIM, Mar 2008]
Uniprot Description
DCLRE1B: 5'-3' exonuclease that plays a central role in telomere maintenance and protection during S-phase. Participates in the protection of telomeres against non-homologous end-joining (NHEJ)- mediated repair, thereby ensuring that telomeres do not fuse. Plays a key role in telomeric loop (T loop) formation by being recruited by TERF2 at the leading end telomeres and by processing leading-end telomeres immediately after their replication via its exonuclease activity: generates 3' single-stranded overhang at the leading end telomeres avoiding blunt leading-end telomeres that are vulnerable to end-joining reactions and expose the telomere end in a manner that activates the DNA repair pathways. Together with TERF2, required to protect telomeres from replicative damage during replication by controlling the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B) needed for telomere replication during fork passage and prevent aberrant telomere topology. Also involved in response to DNA damage: plays a role in response to DNA interstrand cross-links (ICLs) by facilitating double-strand break formation. In case of spindle stress, involved in prophase checkpoint. Defects in DCLRE1B may be a cause of Hoyeraal-Hreidarsson syndrome (HHS). HHS is a multisystem disorder affecting males and is characterized by aplastic anemia, immunodeficiency, microcephaly, cerebellar hypoplasia, and growth retardation. An aberrant splice variant designated Apollo- Delta has been found in a patient with Hoyeraal-Hreidarsson syndrome. Apollo-Delta hampers the proper replication of telomeres, leading to major telomeric dysfunction and cellular senescence, but maintains its DNA interstrand cross-link repair function in the whole genome. Belongs to the DNA repair metallo-beta-lactamase (DRMBL) family.
Protein type: DNA repair, damage; Hydrolase; EC 3.1.-.-
Chromosomal Location of Human Ortholog: 1p13.2
Cellular Component: centrosome; chromosome, telomeric region; cytoplasm; nucleus
Molecular Function: 5'-3' exonuclease activity; protein binding
Biological Process: telomeric loop formation; telomeric 3' overhang formation; DNA repair; telomere maintenance; cell cycle checkpoint; protection from non-homologous end joining at telomere
Research Articles on DCLRE1B
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Product Notes
The DCLRE1B dclre1b (Catalog #AAA820130) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-DCLRE1B Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DCLRE1B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB (1/500 - 1/1000). Researchers should empirically determine the suitability of the DCLRE1B dclre1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DCLRE1B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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