Rabbit anti-Human Cyclin B1 Polyclonal Antibody | anti-CCNB1 antibody

Cyclin B1, phosphorylated (Ser147) (G2/Mitotic-specific Cyclin-B1, CCNB1, CCNB)

Cat. Num. AAA624645

• Gene Names: CCNB1; CCNB
• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Cyclin B1; Polyclonal Antibody; phosphorylated (Ser147) (G2/Mitotic-specific Cyclin-B1; CCNB1; CCNB); Anti -Cyclin B1; anti-CCNB1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Cyclin B1 when phosphorylated at serine 147.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.

• Applicable Applications for anti-CCNB1 antibody: Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunofluorescence and Immunohistochemistry.; Dilution: Immunofluorescence: 1:100-1:200; Immunohistochemistry: 1:50-100
• Immunogen: Synthetic phosphopeptide corresponding to human Cyclin B1 around the phosphorylation site of Serine 147 (A-F-SP-D-V).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-CCNB1 antibody

In mammals, cyclin B associates with inactive p34cdc2 which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition. Ab-2 is excellent for biochemical applications ans co-precipitates cyclin B1 associated p34cdc2.

Product Categories/Family for anti-CCNB1 antibody

Antibodies; Abs to Cyclin

NCBI and Uniprot Product Information

• NCBI GI #: 13518072
• NCBI GeneID: 891
• UniProt Accession #: Q9BPX9; P14635; Q5TZP9; Q9BWU7; Q9BWU8; Q9BWU9; Q9BWV0
• Molecular Weight: 13,039 Da
• NCBI Official Full Name: cyclin B1
• NCBI Official Synonym Full Names: cyclin B1
• NCBI Official Symbol: CCNB1
• NCBI Official Synonym Symbols: CCNB
• NCBI Protein Information: G2/mitotic-specific cyclin-B1; OTTHUMP00000123478; OTTHUMP00000221981; OTTHUMP00000223023; G2/mitotic-specific cyclin B1
• UniProt Protein Name: Cyclin B1
• Protein Family: G2/mitotic-specific cyclin
• UniProt Entry Name: Q9BPX9_HUMAN

NCBI Description

The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq]

Uniprot Description

CCNB1: a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Complexes with cdc2 to form the maturation-promoting factor (MPF). Two alternatively spliced isoforms have been found, a constitutively expressed form and a cell cycle-regulated form that is expressed predominantly during G2/M phase.

Protein type: Cell cycle regulation; Activator

Chromosomal Location of Human Ortholog: 5q12

Cellular Component: nucleoplasm; spindle pole; centrosome; membrane; cytoplasm; cytosol; nucleus

Molecular Function: protein binding; patched binding; histone kinase activity; protein kinase binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; positive regulation of histone phosphorylation; regulation of cell cycle; ventricular cardiac muscle cell development; positive regulation of attachment of spindle microtubules to kinetochore; mitotic nuclear envelope disassembly; positive regulation of mRNA 3'-end processing; positive regulation of mitotic cell cycle; positive regulation of fibroblast proliferation; negative regulation of protein amino acid phosphorylation; tissue regeneration; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; protein complex assembly; G2/M transition of mitotic cell cycle; positive regulation of cardiac muscle cell proliferation; response to drug; in utero embryonic development; oocyte maturation; mitotic metaphase plate congression; response to DDT; gut development; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; cell division; response to mechanical stimulus; spermatogenesis; mitotic cell cycle; regulation of cyclin-dependent protein kinase activity; mitotic spindle stabilization; G1/S transition of mitotic cell cycle