Immunofluorescence (IF)
(Immunofluorescence staining of methanol-fixed Hela cells using Cyclin B1 (Phospho-Ser147) antibody)
Rabbit Cyclin B1 Polyclonal Antibody | anti-CCNB1 antibody
Cyclin B1 antibody
Gene Names
CCNB1; CCNB
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Purified by immunoaffinity chromatography.
Synonyms
Cyclin B1; Polyclonal Antibody; Cyclin B1 antibody; Rabbit polyclonal Cyclin B1 antibody; Polyclonal Cyclin B1 antibody; Anti-Cyclin B1 antibody; Cyclin B 1; Cyclin B 1 antibody; Cyclin B-1; Cyclin B-1 antibody; G2/mitotic-specific cyclin-B1 antibody; Phospho Ser147 antibody; anti-CCNB1 antibody
Host
Rabbit
Clonality
Polyclonal
Purity/Purification
Purified by immunoaffinity chromatography.
Form/Format
Liquid form in PBS buffer (no MG2+ or Ca2+), pH 7.4, 150 mm NaCl,0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
113
Applicable Applications for anti-CCNB1 antibody
Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
IF: 1:100-1:200
IHC: 1:50-1:100
WB: 1:500-1:1000
IHC: 1:50-1:100
WB: 1:500-1:1000
Immunogen
Cyclin B1 antibody was raised in Rabbit using the peptide sequence around phosphorylation site of Serine 147 (A-F-S(p)-D-V) derived from Human Cyclin B1 as the immunogen.
Cross-Reactivity
Human
Preparation and Storage
Store at 4 degree C for short term storage.
Aliquot and store at-20 degree C for long term storage.
Avoid repeated freeze/thaw cycles.
Aliquot and store at-20 degree C for long term storage.
Avoid repeated freeze/thaw cycles.
Immunofluorescence (IF)
(Immunofluorescence staining of methanol-fixed Hela cells using Cyclin B1 (Phospho-Ser147) antibody)
Immunofluorescence (IF)
(Immunofluorescence staining of methanol-fixed Hela cells using Cyclin B1 (Phospho-Ser147) antibody)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Cyclin B1 (Phospho-Ser147) antibody (left) or the same antibody preincubated with with blocking peptide (right))
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Cyclin B1 (Phospho-Ser147) antibody (left) or the same antibody preincubated with with blocking peptide (right))
Related Product Information for anti-CCNB1 antibody
Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase of the cell cycle. The different transcripts result from the use of alternate transcription initiation sites.
Product Categories/Family for anti-CCNB1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
44,102 Da
NCBI Official Full Name
cyclin B1, partial
NCBI Official Synonym Full Names
cyclin B1
NCBI Official Symbol
CCNB1
NCBI Official Synonym Symbols
CCNB
NCBI Protein Information
G2/mitotic-specific cyclin-B1
UniProt Protein Name
G2/mitotic-specific cyclin-B1
Protein Family
UniProt Gene Name
CCNB1
UniProt Synonym Gene Names
CCNB
NCBI Description
The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). The encoded protein is necessary for proper control of the G2/M transition phase of the cell cycle. [provided by RefSeq, Aug 2017]
Uniprot Description
Essential for the control of the cell cycle at the G2/M (mitosis) transition.
Research Articles on CCNB1
Similar Products
Product Notes
The CCNB1 ccnb1 (Catalog #AAA5309633) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Cyclin B1 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB). IF: 1:100-1:200 IHC: 1:50-1:100 WB: 1:500-1:1000. Researchers should empirically determine the suitability of the CCNB1 ccnb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cyclin B1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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