Immunofluorescence (IF)
(Immunofluorescence staining of methanol-fixed HeLa cells using Cyclin B1 (phospho-Ser147) antibody (Red).)
Rabbit Cyclin B1 Polyclonal Antibody | anti-CCNB1 antibody
Cyclin B1 Antibody (Ser147)
Gene Names
CCNB1; CCNB
Applications
Immunohistochemistry, Immunofluorescence
Purity
Affinity Chromatography
Synonyms
Cyclin B1; Polyclonal Antibody; Cyclin B1 Antibody (Ser147); Rabbit Polyclonal Cyclin B1 Antibody (Ser147); Polyclonal Cyclin B1 antibody; Anti-Cyclin B1 antibody; CCNB antibody; CCNB1 antibody; cyclin B1 antibody; G2/mitotic-specific cyclin B1 antibody; G2/mitotic-specific cyclin-B1 antibody; anti-CCNB1 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Specificity
Cyclin B1 antibody (Ser147) detects endogenous levels of Cyclin B1only whenphosphorylated at Serine 147
Purity/Purification
Affinity Chromatography
Form/Format
Supplied in PBS buffer (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% Sodium Azide and 50% Glycerol
Concentration
1 mg/ml (varies by lot)
Sequence Length
113
Applicable Applications for anti-CCNB1 antibody
Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
IHC: 1:50-100
IF: 1:100-1:200
IF: 1:100-1:200
Immunogen
Cyclin B1 antibody was raised in Rabbit using a synthesized phosphopeptide derived from human Cyclin B1 around the phosphorylation site of Serine 147 (A-F-SP-D-V) as the immunogen.
Preparation and Storage
Store at -20 degree C in small aliquots. Avoid repeat freeze/thaw cycles.
Immunofluorescence (IF)
(Immunofluorescence staining of methanol-fixed HeLa cells using Cyclin B1 (phospho-Ser147) antibody (Red).)
Immunofluorescence (IF)
(Immunofluorescence staining of methanol-fixed HeLa cells using Cyclin B1 (phospho-Ser147) antibody (Red).)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Cyclin B1 antibody (Ser147))
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Cyclin B1 antibody (Ser147))
Related Product Information for anti-CCNB1 antibody
Cyclin B1 is a member of the cyclin family of proteins whose levels vary during the cell cycle in order to activate specific cyclin-dependent kinases (CDKs) required for the proper progression through the cell cycle. Cyclin B1 protein begins to increase during G2, peaks in mitosis, and is rapidly degraded before the cell cycle is completed.
Product Categories/Family for anti-CCNB1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
cyclin B1, partial
NCBI Official Synonym Full Names
cyclin B1
NCBI Official Symbol
CCNB1
NCBI Official Synonym Symbols
CCNB
NCBI Protein Information
G2/mitotic-specific cyclin-B1
Protein Family
NCBI Description
The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). The encoded protein is necessary for proper control of the G2/M transition phase of the cell cycle. [provided by RefSeq, Aug 2017]
Research Articles on CCNB1
Similar Products
Product Notes
The CCNB1 (Catalog #AAA5313746) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Cyclin B1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Immunofluorescence (IF). IHC: 1:50-100 IF: 1:100-1:200. Researchers should empirically determine the suitability of the CCNB1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cyclin B1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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