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Typical Testing Data/Standard Curve (for reference only)

Mouse phosphoglycerate dehydrogenase ELISA Kit | Phgdh elisa kit

Mouse D-3-phosphoglycerate dehydrogenase, PHGDH ELISA Kit

Gene Names
Phgdh; A10; PGD; PGAD; PGDH; SERA; 3PGDH; 3-PGDH; 4930479N23
Reactivity
Mouse
Synonyms
phosphoglycerate dehydrogenase; Mouse D-3-phosphoglycerate dehydrogenase; PHGDH ELISA Kit; Phgdh elisa kit
Ordering
For Research Use Only!
Reactivity
Mouse
Specificity
No cross-reaction with other factors.
Samples
Serum, plasma or cell culture supernatant and organizations in the natural and recombinant PHGDH concentration
Assay Type
Sandwich
Detection Range
20 ng/ml-0.312 ng/ml
Sensitivity
0.06 ng/ml.
Intra-assay Precision
? 8%
Inter-assay Precision
? 12%
Preparation and Storage
Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Phgdh elisa kit
Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse PHGDH monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
56,586 Da
NCBI Official Full Name
D-3-phosphoglycerate dehydrogenase
NCBI Official Synonym Full Names
3-phosphoglycerate dehydrogenase
NCBI Official Symbol
Phgdh
NCBI Official Synonym Symbols
A10; PGD; PGAD; PGDH; SERA; 3PGDH; 3-PGDH; 4930479N23
NCBI Protein Information
D-3-phosphoglycerate dehydrogenase; 3-phosphoglycerate dehyrogenase
UniProt Protein Name
D-3-phosphoglycerate dehydrogenase
UniProt Gene Name
Phgdh
UniProt Synonym Gene Names
3-PGDH
UniProt Entry Name
SERA_MOUSE

Uniprot Description

PHGDH: Defects in PHGDH are the cause of phosphoglycerate dehydrogenase deficiency (PHGDH deficiency). It is characterized by congenital microcephaly, psychomotor retardation, and seizures. Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.

Protein type: Cell development/differentiation; EC 1.1.1.95; Amino Acid Metabolism - glycine, serine and threonine; Oxidoreductase

Cellular Component: myelin sheath

Molecular Function: oxidoreductase activity; phosphoglycerate dehydrogenase activity; NAD binding; oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor

Biological Process: neural tube development; glial cell development; glycine metabolic process; metabolic process; gamma-aminobutyric acid metabolic process; glutamine metabolic process; L-serine biosynthetic process; neurogenesis; L-serine metabolic process; regulation of gene expression; spinal cord development; amino acid biosynthetic process; taurine metabolic process; neurite development; threonine metabolic process

Research Articles on Phgdh

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Product Notes

The Mouse Phgdh phgdh (Catalog #AAA2602994) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2602994 ELISA Kit recognizes Mouse Phgdh. It is sometimes possible for the material contained within the vial of "phosphoglycerate dehydrogenase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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