Rabbit anti-Human, Mouse CRIP1 Polyclonal Antibody | anti-CRIP1 antibody

CRIP1, CT (CRIP1, CRIP, CRP1, Cysteine-rich protein 1, Cysteine-rich heart protein, Cysteine-rich intestinal protein)

Cat. Num. AAA6009980

• Gene Names: CRIP1; CRHP; CRIP; CRP1; CRP-1
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: CRIP1; Polyclonal Antibody; CT (CRIP1; CRIP; CRP1; Cysteine-rich protein 1; Cysteine-rich heart protein; Cysteine-rich intestinal protein); Anti -CRIP1; anti-CRIP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human, mouse
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-CRIP1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:50-100; Flow Cytometry: 1:10-50
• Immunogen: CRIP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 48~77 amino acids from the C-terminal region of human CRIP1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CRIP1 Antibody (C-term) in HL-60 cell line lysates (35ug/lane). CRIP1 (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of CRIP1 Antibody (C-term) in mouse bladder tissue lysates (35ug/lane). CRIP1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(CRIP1 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded bladder carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CRIP1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(CRIP1 Antibody (C-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-CRIP1 antibody

Cysteine-rich intestinal protein (CRIP) belongs to the LIM/double zinc finger protein family, members of which include cysteine- and glycine-rich protein-1 (CSRP1; MIM 123876), rhombotin-1 (RBTN1; MIM 186921), rhombotin-2 (RBTN2; MIM 180385), and rhombotin-3 (RBTN3; MIM 180386). CRIP may be involved in intestinal zinc transport.

Product Categories/Family for anti-CRIP1 antibody

Antibodies; Abs to Amino Acids

NCBI and Uniprot Product Information

• NCBI GI #: 4503047
• NCBI GeneID: 1396
• NCBI Accession #: NP_001302.1
• NCBI GenBank Nucleotide #: NM_001311.4
• UniProt Accession #: P50238; Q13628; Q53XY7; Q96J34; H3BPI2
• Molecular Weight: 8,533 Da
• NCBI Official Full Name: cysteine-rich protein 1
• NCBI Official Synonym Full Names: cysteine-rich protein 1 (intestinal)
• NCBI Official Symbol: CRIP1
• NCBI Official Synonym Symbols: CRHP; CRIP; CRP1; CRP-1
• NCBI Protein Information: cysteine-rich protein 1; cysteine-rich heart protein; cysteine-rich intestinal protein
• UniProt Protein Name: Cysteine-rich protein 1
• Protein Family: Cysteine-rich protein
• UniProt Gene Name: CRIP1
• UniProt Synonym Gene Names: CRIP; CRP1; CRP-1; CRHP; hCRHP; CRIP
• UniProt Entry Name: CRIP1_HUMAN

NCBI Description

Cysteine-rich intestinal protein (CRIP) belongs to the LIM/double zinc finger protein family, members of which include cysteine- and glycine-rich protein-1 (CSRP1; MIM 123876), rhombotin-1 (RBTN1; MIM 186921), rhombotin-2 (RBTN2; MIM 180385), and rhombotin-3 (RBTN3; MIM 180386). CRIP may be involved in intestinal zinc transport (Hempe and Cousins, 1991 [PubMed 1946385]).[supplied by OMIM, Mar 2008]

Uniprot Description

CRIP1: a LIM protein which is developmentally regulated in heart. May have a role in zinc absorption and may function as an intracellular zinc transport protein. The CRIP1 gene is hypomethylated in prostate cancer.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 14q32.33

Cellular Component: cytoplasm

Molecular Function: zinc ion binding; AT DNA binding; peptide binding; DNA bending activity

Biological Process: response to organic substance; cell proliferation; DNA damage response, signal transduction resulting in induction of apoptosis; regulation of gene expression; response to zinc ion; heart development; immune response