Rabbit anti-Human, Mouse CRIP1 Polyclonal Antibody | anti-CRIP1 antibody

CRIP1, CT (CRIP1, CRIP, CRP1, Cysteine-rich protein 1, Cysteine-rich heart protein, Cysteine-rich intestinal protein) (APC)

Cat. Num. AAA6288825

• Gene Names: CRIP1; CRHP; CRIP; CRP1; CRP-1
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CRIP1; Polyclonal Antibody; CT (CRIP1; CRIP; CRP1; Cysteine-rich protein 1; Cysteine-rich heart protein; Cysteine-rich intestinal protein) (APC); Cysteine-rich intestinal protein; anti-CRIP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-CRIP1 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: CRIP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 48~77 amino acids from the C-terminal region of human CRIP1.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CRIP1 Antibody (C-term) in HL-60 cell line lysates (35ug/lane). CRIP1 (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of CRIP1 Antibody (C-term) in mouse bladder tissue lysates (35ug/lane). CRIP1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(CRIP1 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded bladder carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CRIP1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(CRIP1 Antibody (C-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-CRIP1 antibody

Cysteine-rich intestinal protein (CRIP) belongs to the LIM/double zinc finger protein family, members of which include cysteine-and glycine-rich protein-1 (CSRP1; MIM 123876), rhombotin-1 (RBTN1; MIM 186921), rhombotin-2 (RBTN2; MIM 180385), and rhombotin-3 (RBTN3; MIM 180386). CRIP may be involved in intestinal zinc transport.

Product Categories/Family for anti-CRIP1 antibody

Antibodies; Amino Acids

References

Garcia-Barcelo, M., et al. Genomics 47(3):419-422(1998). Khoo, C., et al. Protein Expr. Purif. 9(3):379-387(1997).

NCBI and Uniprot Product Information

• NCBI GI #: 4503047
• NCBI GeneID: 1396
• NCBI Accession #: NP_001302.1
• NCBI GenBank Nucleotide #: NM_001311.4
• UniProt Accession #: P50238; Q13628; Q53XY7; Q96J34; H3BPI2
• Molecular Weight: 8,533 Da
• NCBI Official Full Name: cysteine-rich protein 1
• NCBI Official Synonym Full Names: cysteine-rich protein 1 (intestinal)
• NCBI Official Symbol: CRIP1
• NCBI Official Synonym Symbols: CRHP; CRIP; CRP1; CRP-1
• NCBI Protein Information: cysteine-rich protein 1; cysteine-rich heart protein; cysteine-rich intestinal protein
• UniProt Protein Name: Cysteine-rich protein 1
• Protein Family: Cysteine-rich protein
• UniProt Gene Name: CRIP1
• UniProt Synonym Gene Names: CRIP; CRP1; CRP-1; CRHP; hCRHP; CRIP
• UniProt Entry Name: CRIP1_HUMAN

NCBI Description

Cysteine-rich intestinal protein (CRIP) belongs to the LIM/double zinc finger protein family, members of which include cysteine- and glycine-rich protein-1 (CSRP1; MIM 123876), rhombotin-1 (RBTN1; MIM 186921), rhombotin-2 (RBTN2; MIM 180385), and rhombotin-3 (RBTN3; MIM 180386). CRIP may be involved in intestinal zinc transport (Hempe and Cousins, 1991 [PubMed 1946385]).[supplied by OMIM, Mar 2008]

Uniprot Description

CRIP1: a LIM protein which is developmentally regulated in heart. May have a role in zinc absorption and may function as an intracellular zinc transport protein. The CRIP1 gene is hypomethylated in prostate cancer.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 14q32.33

Cellular Component: cytoplasm

Molecular Function: AT DNA binding; zinc ion binding; DNA bending activity; peptide binding

Biological Process: response to organic substance; cell proliferation; DNA damage response, signal transduction resulting in induction of apoptosis; response to zinc ion; regulation of gene expression; heart development; immune response