Western Blot (WB)
(Western Blot (WB) analysis of ULK1 (S757) polyclonal antibody at 1:500 dilutionLane1:L02 whole cell lysate (40ug) Lane2:Panc1 whole cell lysate (40ug) Lane3:C6 whole cell lysate (40ug) Lane4:AML-12 whole cell lysate (40ug))
Rabbit anti-Human VPS34 Polyclonal Antibody | anti-VPS34 antibody
VPS34 (Phospho-Ser249) Polyclonal Antibody
Reactivity
Human
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
VPS34; Polyclonal Antibody; VPS34 (Phospho-Ser249) Polyclonal Antibody; Serine/threonine-protein kinase ULK1; Autophagy-related protein 1 homolog; ATG1; Unc-51-like kinase 1; ULK1; KIAA0722; anti-VPS34 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
ULK1 (S757) polyclonal antibody detects endogenous levels of ULK1 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Applicable Applications for anti-VPS34 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:1000
Immunogen
Synthetic peptide, corresponding to amino acids 700-800 of Human ULK1.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of ULK1 (S757) polyclonal antibody at 1:500 dilutionLane1:L02 whole cell lysate (40ug) Lane2:Panc1 whole cell lysate (40ug) Lane3:C6 whole cell lysate (40ug) Lane4:AML-12 whole cell lysate (40ug))
Western Blot (WB)
(Western Blot (WB) analysis of ULK1 (S757) polyclonal antibody at 1:500 dilutionLane1:L02 whole cell lysate (40ug) Lane2:Panc1 whole cell lysate (40ug) Lane3:C6 whole cell lysate (40ug) Lane4:AML-12 whole cell lysate (40ug))
Related Product Information for anti-VPS34 antibody
ULK1 and ULK2 (for UNC-51-like kinase) encode similar amino-terminal serine/threonine kinase domains, a proline/serine-rich (PS) domain, and a species conserved carboxyl-terminal domain. Both share homology with the UNC-51 kinase from Caenorhabditis elegans and the APG1 kinase in yeast, which are involved in axonal extension and growth, and autophagy, respectively. ULK1 and ULK2 are thought to auto-phosphorylate the PS domain in vitro, and the significant homology among vertebrates suggest that ULK1 and ULK2 are involved in the regulation of fundamental biological processes.
NCBI and Uniprot Product Information
UniProt Accession #
Molecular Weight
~ 150kDa
Protein Family
Similar Products
Product Notes
The VPS34 (Catalog #AAA3000261) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The VPS34 (Phospho-Ser249) Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's VPS34 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:1000. Researchers should empirically determine the suitability of the VPS34 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VPS34, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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