Rabbit anti-Human CRADD Polyclonal Antibody | anti-CRADD antibody

CRADD, ID (CRADD, RAIDD, Death domain-containing protein CRADD, Caspase and RIP adapter with death domain, RIP-associated protein with a death domain) (PE)

Cat. Num. AAA6288592

• Gene Names: CRADD; MRT34; RAIDD
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CRADD; Polyclonal Antibody; ID (CRADD; RAIDD; Death domain-containing protein CRADD; Caspase and RIP adapter with death domain; RIP-associated protein with a death domain) (PE); RIP-associated protein with a death domain; anti-CRADD antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-CRADD antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: CRADD antibody is generated from rabbits immunized with a KLH conjugated synthetic between 68-98 amino acids selected from the Central region of human CRADD.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(CRADD Antibody (Center) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the CRADD antibody detected the CRADD protein (arrow).)

Related Product Information for anti-CRADD antibody

The protein encoded by this gene is a death domain (CARD/DD)-containing protein and has been shown to induce cell apoptosis. Through its CARD domain, this protein interacts with, and thus recruits, caspase 2/ICH1 to the cell death signal transduction complex that includes tumor necrosis factor receptor 1 (TNFR1A), RIPK1/RIP kinase, and numbers of other CARD domain-containing proteins.

Product Categories/Family for anti-CRADD antibody

Antibodies; Apoptosis; Caspase

References

Shimada, M., et al. Hum. Genet. 128(4):433-441(2010). Jang, T.H., et al. Biochim. Biophys. Acta 1804(7):1557-1563(2010). Davila, S., et al. Genes Immun. 11(3):232-238(2010). Zhao, J., et al. BMC Med. Genet. 11, 96 (2010): Heikaus, S., et al. Cell. Oncol. 32 (1-2), 29-42 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 4503031
• NCBI GeneID: 8738
• NCBI Accession #: NP_003796.1
• NCBI GenBank Nucleotide #: NM_003805.3
• UniProt Accession #: P78560; B7Z2Q5
• Molecular Weight: 11,826 Da
• NCBI Official Full Name: death domain-containing protein CRADD
• NCBI Official Synonym Full Names: CASP2 and RIPK1 domain containing adaptor with death domain
• NCBI Official Symbol: CRADD
• NCBI Official Synonym Symbols: MRT34; RAIDD
• NCBI Protein Information: death domain-containing protein CRADD; RIP-associated ICH1/CED3-homologous protein with death domain; RIP-associated protein with a death domain; caspase and RIP adaptor with death domain; death adaptor molecule RAIDD
• UniProt Protein Name: Death domain-containing protein CRADD
• Protein Family: Death domain-containing protein
• UniProt Gene Name: CRADD
• UniProt Synonym Gene Names: RAIDD
• UniProt Entry Name: CRADD_HUMAN

NCBI Description

The protein encoded by this gene is a death domain (CARD/DD)-containing protein and has been shown to induce cell apoptosis. Through its CARD domain, this protein interacts with, and thus recruits, caspase 2/ICH1 to the cell death signal transduction complex that includes tumor necrosis factor receptor 1 (TNFR1A), RIPK1/RIP kinase, and numbers of other CARD domain-containing proteins. [provided by RefSeq, Jul 2008]

Uniprot Description

CRADD: Apoptotic adaptor molecule specific for caspase-2 and FASL/TNF receptor-interacting protein RIP. In the presence of RIP and TRADD, CRADD recruits caspase-2 to the TNFR-1 signalling complex. Defects in CRADD are the cause of mental retardation autosomal recessive type 34 (MRT34). A disorder characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. MRT34 is a non- syndromic form. Affected individuals have mildly delayed development and significantly impaired cognitive function, precluding independent living and self-care. Speech is rudimentary, but articulate; autism is not present.

Protein type: Adaptor/scaffold; Apoptosis

Chromosomal Location of Human Ortholog: 12q21.33-q23.1

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding, bridging; protein binding; protease binding

Biological Process: caspase activation; induction of apoptosis via death domain receptors; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest

Disease: Mental Retardation, Autosomal Recessive 34