Rabbit ERO1L Polyclonal Antibody | anti-ERO1L antibody

ERO1L Antibody

Cat. Num. AAA9611735

• Gene Names: ERO1A; ERO1L; ERO1-L; ERO1LA; Ero1alpha; ERO1-alpha; ERO1-L-alpha
• Reactivity: Human, Mouse, Rat; Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
• Applications: Western Blot
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: ERO1L; Polyclonal Antibody; ERO1L Antibody; Endoplasmic oxidoreductin 1 like protein; Endoplasmic oxidoreductin-1-like protein; Endoplasmic reticulum oxidoreductase 1 alpha; Endoplasmic reticulum oxidoreductin 1-like; ERO1 alpha; ERO1 L; ERO1 Lalpha; ERO1 like protein alpha; ERO1-alpha; ERO1-L; ERO1-L-alpha; ERO1-like (S. cerevisiae); ERO1-like alpha; ERO1-like protein alpha; ERO1-like; S. cerevisiae; homolog of; alpha; ERO1A; ERO1A_HUMAN; ERO1LA; Oxidoreductin 1 Lalpha; Oxidoreductin-1-L-alpha; PRO865; UNQ434; anti-ERO1L antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ERO1L Antibody detects endogenous levels of ERO1L.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 468

• Applicable Applications for anti-ERO1L antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: A synthesized peptide derived from human ERO1L.
• Tissue Specificity: Widely expressed at low level. Expressed at high level in upper digestive tract. Highly expressed in esophagus. Weakly expressed in stomach and duodenum.
• Fragment: Fab Fragment
• Conjugate: Unconjugated
• Subcellular Location: Endoplasmic Reticulum
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from A431 cells, using ERO1L Antibody. The lane on the left was treated with blocking peptide.)

Related Product Information for anti-ERO1L antibody

Description: Oxidoreductase involved in disulfide bond formation in the endoplasmic reticulum. Efficiently reoxidizes P4HB/PDI, the enzyme catalyzing protein disulfide formation, in order to allow P4HB to sustain additional rounds of disulfide formation. Following P4HB reoxidation, passes its electrons to molecular oxygen via FAD, leading to the production of reactive oxygen species (ROS) in the cell. Required for the proper folding of immunoglobulins. Involved in the release of the unfolded cholera toxin from reduced P4HB/PDI in case of infection by V.cholerae, thereby playing a role in retrotranslocation of the toxin. Plays an important role in ER stress-induced, CHOP-dependent apoptosis by activating the inositol 1,4,5-trisphosphate receptor IP3R1.

Post Translational Modifications: N-glycosylated. The Cys-94/Cys-99 and Cys-394/Cys-397 disulfide bonds constitute the redox-active center. The Cys-94/Cys-99 disulfide bond may accept electron from P4HB and funnel them to the active site disulfide Cys-394/Cys-397 (By similarity). The regulatory Cys-99/Cys-104 disulfide bond stabilizes the other regulatory bond Cys-94/Cys-131 (PubMed:23027870).

Subunit Structure: Predominantly monomer. May function both as a monomer and a homodimer. Interacts with PDILT.

Similarity: Belongs to the EROs family.

NCBI and Uniprot Product Information

• NCBI GI #: 7657069
• NCBI GeneID: 30001
• NCBI Accession #: NP_055399
• NCBI GenBank Nucleotide #: NP_055399.1
• UniProt Accession #: Q96HE7
• Molecular Weight: Observed: 54 kDa; Predicted: 55 kDa
• NCBI Official Full Name: ERO1-like protein alpha
• NCBI Official Synonym Full Names: endoplasmic reticulum oxidoreductase 1 alpha
• NCBI Official Symbol: ERO1A
• NCBI Official Synonym Symbols: ERO1L; ERO1-L; ERO1LA; Ero1alpha; ERO1-alpha; ERO1-L-alpha
• NCBI Protein Information: ERO1-like protein alpha
• UniProt Protein Name: ERO1-like protein alpha
• Protein Family: Ero1-like protein
• UniProt Gene Name: ERO1A
• UniProt Synonym Gene Names: ERO1-L; ERO1-L-alpha

Uniprot Description

Oxidoreductase involved in disulfide bond formation in the endoplasmic reticulum. Efficiently reoxidizes P4HB/PDI, the enzyme catalyzing protein disulfide formation, in order to allow P4HB to sustain additional rounds of disulfide formation. Following P4HB reoxidation, passes its electrons to molecular oxygen via FAD, leading to the production of reactive oxygen species (ROS) in the cell. Required for the proper folding of immunoglobulins. Involved in the release of the unfolded cholera toxin from reduced P4HB/PDI in case of infection by V.cholerae, thereby playing a role in retrotranslocation of the toxin. Plays an important role in ER stress-induced, CHOP-dependent apoptosis by activating the inositol 1,4,5-trisphosphate receptor IP3R1.