Human Coronin 1A (CORO1A) ELISA Kit | CORO1A elisa kit

Human Coronin 1A (CORO1A) ELISA Kit

Cat. Num. AAA2020522

• Gene Names: CORO1A; p57; IMD8; TACO; CLABP; HCORO1; CLIPINA
• Reactivity: Human
• Synonyms: Coronin 1A (CORO1A); Human Coronin 1A (CORO1A) ELISA Kit; CLABP; p57; CLIPINA; HCORO1; TACO; Coronin-1; Coronin; Actin-Binding Protein; 1A; Coronin-like protein A; Coronin-like protein p57; Tryptophan aspartate-containing coat protein; CORO1A elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Coronin 1A (CORO1A).; No significant cross-reactivity or interference between Coronin 1A (CORO1A) and analogues was observed.
• Sequence Length: 461

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.119ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Coronin 1A (CORO1A) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Coronin 1A (CORO1A) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CORO1A elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Coronin 1A (CORO1A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Coronin 1A (CORO1A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Coronin 1A (CORO1A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Coronin 1A (CORO1A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for CORO1A elisa kit

Metabolic pathway

NCBI and Uniprot Product Information

• NCBI GI #: 5902134
• NCBI GeneID: 11151
• NCBI Accession #: NP_009005.1
• NCBI GenBank Nucleotide #: NM_007074.3
• UniProt Accession #: P31146; Q2YD73; B2RBL1
• Molecular Weight: 51,026 Da
• NCBI Official Full Name: coronin-1A
• NCBI Official Synonym Full Names: coronin, actin binding protein, 1A
• NCBI Official Symbol: CORO1A
• NCBI Official Synonym Symbols: p57; IMD8; TACO; CLABP; HCORO1; CLIPINA
• NCBI Protein Information: coronin-1A
• UniProt Protein Name: Coronin-1A
• Protein Family: Coronin
• UniProt Gene Name: CORO1A
• UniProt Synonym Gene Names: CORO1; Clipin-A; TACO
• UniProt Entry Name: COR1A_HUMAN

NCBI Description

This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. Alternative splicing results in multiple transcript variants. A related pseudogene has been defined on chromosome 16. [provided by RefSeq, Sep 2010]

Uniprot Description

coronin 1A: May be a crucial component of the cytoskeleton of highly motile cells, functioning both in the invagination of large pieces of plasma membrane, as well as in forming protrusions of the plasma membrane involved in cell locomotion. In mycobacteria- infected cells, its retention on the phagosomal membrane prevents fusion between phagosomes and lysosomes. Belongs to the WD repeat coronin family.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: phagocytic vesicle membrane; cortical actin cytoskeleton; protein complex; membrane; axon; lamellipodium; cytoplasm; early endosome; plasma membrane; immunological synapse; actin filament; phagocytic vesicle; intercellular junction; nucleus; phagocytic cup

Molecular Function: actin filament binding; protein C-terminus binding; protein binding; protein homodimerization activity; cytoskeletal protein binding; phosphoinositide 3-kinase binding

Biological Process: negative regulation of vesicle fusion; leukocyte chemotaxis; actin filament organization; regulation of release of sequestered calcium ion into cytosol; cell-substrate adhesion; phagocytosis; uropod organization and biogenesis; phagolysosome formation; regulation of cell shape; regulation of actin filament polymerization; positive chemotaxis; homeostasis of number of cells within a tissue; negative regulation of actin nucleation; calcium ion transport; innate immune response; positive regulation of T cell proliferation; T cell homeostasis; negative regulation of neuron apoptosis; actin cytoskeleton organization and biogenesis; cell motility; positive regulation of cell migration

Disease: Immunodeficiency 8