Rabbit anti-Mouse CIDE-A Polyclonal Antibody | anti-CIDEA antibody

CIDE-A Antibody

Cat. Num. AAA150497

• Gene Names: CIDEA; CIDE-A
• Reactivity: Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: CIDE-A Antibody is affinity chromatography purified via peptide column.
• Synonyms: CIDE-A; Polyclonal Antibody; CIDE-A Antibody; Cell death activator CIDE-A; Cell death-inducing DFFA-like effector A; cell death-inducing DFFA-like effector a; anti-CIDEA antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: It has no cross activity to CIDE-B.
• Purity/Purification: CIDE-A Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 660

• Applicable Applications for anti-CIDEA antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: CIDE-A antibody can be used for detection of CIDE-A by Western blot at 0.5 mug/mL. Antibody can also be used for immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: CIDE-A antibody was raised against an 18 amino acid peptide near the carboxy terminus of human CIDE-A.
• Buffer: CIDE-A Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: CIDE-A antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of CIDE-A in mouse heart tissue lysate with CIDE-A antibody at 1:500 dilution.)

Immunohistochemistry (IHC)

(Immunohistochemistry of CIDE-A in mouse heart tissue with CIDE-A antibody at 5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of CIDE-A in Mouse Heart cells with CIDE-A antibody at 20 ug/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of CIDE-A in human brain tissue with CIDE-A antibody at 2.5 μg/ml.)

Immunofluorescence (IF)

(Immunofluorescence of CIDE-A in human brain tissue with CIDE A antibody at 20 μg/mL.Green: CIDE-A Antibody (2089)Blue: DAPI staining)

Immunohistochemistry (IHC)

(Immunohistochemistry of CIDE-A in human brain tissue with CIDE A antibody at 2.5 μg/mL.)

Related Product Information for anti-CIDEA antibody

CIDE-A Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. DFF45/ICAD has been identified as inhibitor of caspase activated DNase DFF40/CAD. DFF45 related proteins CIDE-A and CIDE-B (for cell death-inducing DFF-like effector A and B) were recently identified. CIDE contains a new type of domain termed CIDE-N, which has high homology with the regulatory domains of DFF45/ICAD and DFF40/CAD. Expression of CIDE-A induces DNA fragmentation and activates apoptosis, which is inhibited by DFF45. CIDE-A is a DFF45-inhibitable effector that promotes cell death and DNA fragmentation. CIDE-A is expressed in many tissues.

NCBI and Uniprot Product Information

• NCBI GI #: 3114595
• NCBI GeneID: 1149
• UniProt Accession #: O60543; Q6UPR7; B0YIY7
• Molecular Weight: 25 kDa
• NCBI Official Full Name: Homo sapiens cell death activator CIDE-A (CIDE-A) mRNA, complete cds
• NCBI Official Synonym Full Names: cell death-inducing DFFA-like effector a
• NCBI Official Symbol: CIDEA
• NCBI Official Synonym Symbols: CIDE-A
• NCBI Protein Information: cell death activator CIDE-A
• UniProt Protein Name: Cell death activator CIDE-A
• Protein Family: Cell death activator
• UniProt Gene Name: CIDEA
• UniProt Entry Name: CIDEA_HUMAN

NCBI Description

This gene encodes the homolog of the mouse protein Cidea that has been shown to activate apoptosis. This activation of apoptosis is inhibited by the DNA fragmentation factor DFF45 but not by caspase inhibitors. Mice that lack functional Cidea have higher metabolic rates, higher lipolysis in brown adipose tissue and higher core body temperatures when subjected to cold. These mice are also resistant to diet-induced obesity and diabetes. This suggests that in mice this gene product plays a role in thermogenesis and lipolysis. Alternatively spliced transcripts have been identified. [provided by RefSeq, Aug 2010]

Uniprot Description

CIDEA: Acts as a CEBPB coactivator in mammary epithelial cells to control the expression of a subset of CEBPB downstream target genes, including ID2, IGF1, PRLR, SOCS1, SOCS3, XDH, but not casein. By interacting with CEBPB, strengthens the association of CEBPB with the XDH promoter, increases histone acetylation and dissociates HDAC1 from the promoter. Binds to lipid droplets and regulates their enlargement, thereby restricting lipolysis and favoring storage. At focal contact sites between lipid droplets, promotes directional net neutral lipid transfer from the smaller to larger lipid droplets. The transfer direction may be driven by the internal pressure difference between the contacting lipid droplet pair and occurs at a lower rate than that promoted by CIDEC. When overexpressed, induces apoptosis. The physiological significance of its role in apoptosis is unclear. In omental and subcutaneous adipose tissue of obese patients matched for BMI, expression levels correlate with insulin sensitivity. Expression is increased 5-6 fold in the group of patients with high insulin sensitivity, compared to the insulin- resistant group. This observation is consistent with the idea that triglyceride storage in adipocytes plays an important role in sequestering triglycerides and fatty acids away from the circulation and peripheral tissues, thus enhancing insulin sensitivity in liver and muscle.

Protein type: Apoptosis; Mitochondrial

Chromosomal Location of Human Ortholog: 18p11.21|18

Cellular Component: mitochondrial envelope; mitochondrion; cytoplasm; lipid particle; nucleus

Molecular Function: protein homodimerization activity

Biological Process: cell death; regulation of apoptosis; sequestering of lipid; regulation of transcription, DNA-dependent; transcription, DNA-dependent; negative regulation of lipid catabolic process; apoptosis; negative regulation of cytokine secretion; lipid metabolic process; negative regulation of tumor necrosis factor production; thermoregulation; negative regulation of transforming growth factor beta receptor signaling pathway