Rabbit anti-Human, Mouse RELT Polyclonal Antibody | anti-RELT antibody

RELT Antibody

Cat. Num. AAA9609029

• Gene Names: RELT; TRLT; TNFRSF19L
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: RELT; Polyclonal Antibody; RELT Antibody; Receptor expressed in lymphoid tissues; Relt; TNFRSF19L; TR19L_HUMAN; Tumor necrosis factor receptor superfamily member 19L; anti-RELT antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: RELT antibody detects endogenous levels of total RELT
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 430

• Applicable Applications for anti-RELT antibody: Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
• Application Notes: WB: 1:1000-3000; IF/ICC: 1:100-1:500
• Immunogen: A synthesized peptide derived from human RELT
• Subcellular Location: Cell Membrane. Cytoplasm.
• Tissue Specificity: Highest levels are in spleen, lymph node, thymus, peripheral blood leukocytes, bone marrow and fetal liver. Very low levels in skeletal muscle, testis and colon. Not detected in brain, kidney and pancreas.
• Predicted Cross Reactivity: Bovine, Sheep, Rabbit, Dog
• Similarity: Bovine (100%), Sheep (100%), Rabbit (90%), Dog (100%)
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of RELT using HuvEc whole cell lysates)

Immunofluorescene (IF)

(MBS9609029 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

Related Product Information for anti-RELT antibody

Function: Mediates activation of NF-kappa-B. May play a role in T-cell activation.
Subunit Structure: Associates with TRAF1. Interacts with RELL1, RELL2 and OXSR1.
Post-translational Modifications: Phosphorylated in vitro by OXSR1.
Similarity: Belongs to the RELT family.

NCBI and Uniprot Product Information

• NCBI GI #: 21361873
• NCBI GeneID: 84957
• NCBI Accession #: NP_116260.2
• NCBI GenBank Nucleotide #: NM_032871.3
• UniProt Accession #: Q969Z4; Q86V34; Q96JU1; Q9BUX7
• Molecular Weight: Observed: 46 kDa; Predicted: 47 kDa
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 19L
• NCBI Official Synonym Full Names: RELT, TNF receptor
• NCBI Official Symbol: RELT
• NCBI Official Synonym Symbols: TRLT; TNFRSF19L
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 19L
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 19L
• Protein Family: RELT-like protein
• UniProt Gene Name: RELT
• UniProt Synonym Gene Names: TNFRSF19L

NCBI Description

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is especially abundant in hematologic tissues. It has been shown to activate the NF-kappaB pathway and selectively bind TNF receptor-associated factor 1 (TRAF1). This receptor is capable of stimulating T-cell proliferation in the presence of CD3 signaling, which suggests its regulatory role in immune response. Two alternatively spliced transcript variants of this gene encoding the same protein have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

May play a role in apoptosis (PubMed:28688764, PubMed:19969290). Induces activation of MAPK14/p38 and MAPK8/JNK MAPK cascades, when overexpressed (PubMed:16530727).