Rabbit anti-Human CIDE-A Polyclonal Antibody | anti-CIDE-A antibody

CIDE-A Antibody

Cat. Num. AAA9405305

• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity chromatography purified via peptide column
• Synonyms: CIDE-A; Polyclonal Antibody; CIDE-A Antibody; anti-CIDE-A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: It has no cross activity to CIDE-B.
• Purity/Purification: Affinity chromatography purified via peptide column
• Form/Format: Supplied in PBS containing 0.02% sodium azide.
• Sequence Length: 660

• Applicable Applications for anti-CIDE-A antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Immunogen Type: Peptide
• Immunogen Description: Raised against a peptide corresponding to amino acids 200 to 217 of human CIDE-A.
• Target Name: CIDE-A
• Preparation and Storage: Can be stored at -20 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of CIDE-A in human brain tissue lysate in the absence (A) or presence (B) of peptide (2085P) with CIDE-A antibody at 1:2000 dilution.)

Immunohistochemistry (IHC)

(Immunohistochemistry of CIDE-A in human brain tissue with CIDE-A antibody at 5 ug/mL.)

Related Product Information for anti-CIDE-A antibody

Apoptosis is related to many diseases and induced by a family of cell death receptors. Cell death signals are transduced by DD-, DED-, or CARD-containing molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase DFF40/CAD, which is chaperoned and inhibited by DFF45/ICAD. DFF45 related proteins CIDE-A and CIDE-B (for cell death-inducing DFF-like effector A and B) were recently identified. CIDE contains a new type of domain termed CIDE-N, which has high homology with the regulatory domains of DFF45/ICAD and DFF40/CAD. Expression of CIDE-A induces DNA fragmentation and activates apoptosis, which is inhibited by DFF45. CIDE-A is expressed in many tissues.

Product Categories/Family for anti-CIDE-A antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 3114595
• Molecular Weight: 24,687 Da
• NCBI Official Full Name: Homo sapiens cell death activator CIDE-A (CIDE-A) mRNA, complete cds
• UniProt Protein Name: Cell death activator CIDE-A
• UniProt Gene Name: CIDEA
• UniProt Entry Name: CIDEA_HUMAN

Uniprot Description

CIDEA: Acts as a CEBPB coactivator in mammary epithelial cells to control the expression of a subset of CEBPB downstream target genes, including ID2, IGF1, PRLR, SOCS1, SOCS3, XDH, but not casein. By interacting with CEBPB, strengthens the association of CEBPB with the XDH promoter, increases histone acetylation and dissociates HDAC1 from the promoter. Binds to lipid droplets and regulates their enlargement, thereby restricting lipolysis and favoring storage. At focal contact sites between lipid droplets, promotes directional net neutral lipid transfer from the smaller to larger lipid droplets. The transfer direction may be driven by the internal pressure difference between the contacting lipid droplet pair and occurs at a lower rate than that promoted by CIDEC. When overexpressed, induces apoptosis. The physiological significance of its role in apoptosis is unclear. In omental and subcutaneous adipose tissue of obese patients matched for BMI, expression levels correlate with insulin sensitivity. Expression is increased 5-6 fold in the group of patients with high insulin sensitivity, compared to the insulin- resistant group. This observation is consistent with the idea that triglyceride storage in adipocytes plays an important role in sequestering triglycerides and fatty acids away from the circulation and peripheral tissues, thus enhancing insulin sensitivity in liver and muscle.

Protein type: Mitochondrial; Apoptosis

Chromosomal Location of Human Ortholog: 18p11.21|18

Cellular Component: mitochondrial envelope; mitochondrion; cytoplasm; lipid particle; nucleus

Molecular Function: protein homodimerization activity

Biological Process: regulation of apoptosis; cell death; sequestering of lipid; regulation of transcription, DNA-dependent; transcription, DNA-dependent; negative regulation of lipid catabolic process; apoptosis; negative regulation of cytokine secretion; lipid metabolic process; thermoregulation; negative regulation of tumor necrosis factor production; negative regulation of transforming growth factor beta receptor signaling pathway