Rabbit anti-Human CIDEA / CIDE-A Polyclonal Antibody | anti-CIDEA antibody

Rabbit Polyclonal to Human CIDEA / CIDE-A

Cat. Num. AAA240969

• Gene Names: CIDEA; CIDE-A
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Immunoaffinity Purified
• Synonyms: CIDEA / CIDE-A; Polyclonal Antibody; Rabbit Polyclonal to Human CIDEA / CIDE-A; Anti-CIDEA / CIDE-A Antibody (aa200-217) IHC-plus; CIDEA; CIDE-A; Cell death activator CIDE-A; Human CIDEA; anti-CIDEA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: peptide corresponding to amino acids 200 to 217 of human CIDE-A
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.02% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 219

• Applicable Applications for anti-CIDEA antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
• Application Notes: IHC-P (10 ug/ml), WB (1:1000 - 1:2000); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib...
• Target Species: Human
• Immunogen Description: Synthetic peptide from human CIDEA.
• Immunogen Type: Synthetic peptide
• Immunogen: CIDEA / CIDE-A antibody was raised against synthetic peptide from human CIDEA.
• Antigen Modification: aa200-217
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.

Immunohistochemistry (IHC)

(Anti-CIDE A antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of CIDE-A in Human Brain cells with CIDE-A antibody at 20 ug/ml.)

Western Blot (WB)

(Western blot of CIDE-A in human brain tissue lysate in the absence (A) or presence (B) of blocking peptide with CIDE-A antibody at 1:2000 dilution.)

Related Product Information for anti-CIDEA antibody

Apoptosis is related to many diseases and induced by a family of cell death receptors. Cell death signals are transduced by DD-, DED-, or CARD-containing molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase DFF40/CAD, which is chaperoned and inhibited by DFF45/ICAD. DFF45 related proteins CIDE-A and CIDE-B (for cell death-inducing DFF-like effector A and B) were recently identified.

Product Categories/Family for anti-CIDEA antibody

Family: Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 4557465
• NCBI GeneID: 1149
• NCBI Accession #: NP_001270.1
• NCBI GenBank Nucleotide #: NM_001279.3
• UniProt Accession #: O60543; Q6UPR7; B0YIY7
• Molecular Weight: 24,687 Da
• NCBI Official Full Name: cell death activator CIDE-A isoform 1
• NCBI Official Synonym Full Names: cell death-inducing DFFA-like effector a
• NCBI Official Symbol: CIDEA
• NCBI Official Synonym Symbols: CIDE-A
• NCBI Protein Information: cell death activator CIDE-A
• UniProt Protein Name: Cell death activator CIDE-A
• Protein Family: Cell death activator
• UniProt Gene Name: CIDEA
• UniProt Entry Name: CIDEA_HUMAN

NCBI Description

This gene encodes the homolog of the mouse protein Cidea that has been shown to activate apoptosis. This activation of apoptosis is inhibited by the DNA fragmentation factor DFF45 but not by caspase inhibitors. Mice that lack functional Cidea have higher metabolic rates, higher lipolysis in brown adipose tissue and higher core body temperatures when subjected to cold. These mice are also resistant to diet-induced obesity and diabetes. This suggests that in mice this gene product plays a role in thermogenesis and lipolysis. Alternatively spliced transcripts have been identified. [provided by RefSeq, Aug 2010]

Uniprot Description

CIDEA: Acts as a CEBPB coactivator in mammary epithelial cells to control the expression of a subset of CEBPB downstream target genes, including ID2, IGF1, PRLR, SOCS1, SOCS3, XDH, but not casein. By interacting with CEBPB, strengthens the association of CEBPB with the XDH promoter, increases histone acetylation and dissociates HDAC1 from the promoter. Binds to lipid droplets and regulates their enlargement, thereby restricting lipolysis and favoring storage. At focal contact sites between lipid droplets, promotes directional net neutral lipid transfer from the smaller to larger lipid droplets. The transfer direction may be driven by the internal pressure difference between the contacting lipid droplet pair and occurs at a lower rate than that promoted by CIDEC. When overexpressed, induces apoptosis. The physiological significance of its role in apoptosis is unclear. In omental and subcutaneous adipose tissue of obese patients matched for BMI, expression levels correlate with insulin sensitivity. Expression is increased 5-6 fold in the group of patients with high insulin sensitivity, compared to the insulin- resistant group. This observation is consistent with the idea that triglyceride storage in adipocytes plays an important role in sequestering triglycerides and fatty acids away from the circulation and peripheral tissues, thus enhancing insulin sensitivity in liver and muscle.

Protein type: Apoptosis; Mitochondrial

Chromosomal Location of Human Ortholog: 18p11.21|18

Cellular Component: mitochondrial envelope; mitochondrion; cytoplasm; lipid particle; nucleus

Molecular Function: protein homodimerization activity

Biological Process: regulation of apoptosis; cell death; sequestering of lipid; negative regulation of lipid catabolic process; regulation of transcription, DNA-dependent; transcription, DNA-dependent; apoptosis; negative regulation of cytokine secretion; lipid metabolic process; negative regulation of tumor necrosis factor production; thermoregulation; negative regulation of transforming growth factor beta receptor signaling pathway