Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Mouse Checkpoint Homolog (CHEK1) Polyclonal Antibody | anti-CHEK1 antibody
Polyclonal Antibody to Checkpoint Homolog (CHEK1)
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- CGIVLTA MLAGELPWDQ PSDSCQEYSD WKEKKTYLNP WKKIDSAPLA LLHKILVETP SARITIPDIK KDRWYNKPLN RGAKRPRATS GGMSESSSGF SKHIHSNLDF SPVNNGSSEE TVKFSSSQPE PRTGLSLWDT GPSNVDKLVQ GISFSQPTCP EHMLVNSQLL GTPGSSQNPW QRLVKRMTRF FTKLDADKSY
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DABstainingonIHC-P.Samples:MouseTissue))
Immunohistochemistry (IHC)
(DABstainingonIHC-P.Samples:MouseTissue))
NCBI and Uniprot Product Information
Uniprot Description
Chk1: a protein kinase of the CAMKL family. Required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. Plays an important role in both S and G2 checkpoints, embryonic development and tumor suppression. Phosphorylated and activated by ATM/ATR following DNA damage, it binds to single-stranded DNA and DNA ends and has a role in the repair of double strand breaks. Activated Chk1 can phosphorylate and inactivate cdc25C via phosphorylation and 14-3-3 binding, blocking the activation of cdc2 and transition into M-phase. Other substrates include p53. Implicated in resistance to apoptosis in response to chemotherapy. Inhibitors under development to chemosensitize tumors. Somatic mutations found in stomach tumors, and in colon and endometrial tumors, where CHK1 may be a target of microsatellite instability. Inhibitors: SB218078, UNC-01.
Protein type: CAMK group; CAMKL family; CHK1 subfamily; EC 2.7.11.1; Kinase, protein; Protein kinase, CAMK; Protein kinase, Ser/Thr (non-receptor); Tumor suppressor
Chromosomal Location of Human Ortholog: 9|9 A4
Cellular Component: centrosome; chromatin; chromosome, telomeric region; condensed nuclear chromosome; cytoplasm; cytoskeleton; extracellular space; intracellular membrane-bound organelle; nucleoplasm; nucleus; protein complex; replication fork
Molecular Function: ATP binding; kinase activity; nucleotide binding; protein binding; protein domain specific binding; protein kinase activity; protein serine/threonine kinase activity; transferase activity
Biological Process: apoptosis; apoptotic process involved in development; cell cycle; cellular response to DNA damage stimulus; chromatin-mediated maintenance of transcription; DNA damage checkpoint; DNA damage induced protein phosphorylation; DNA repair; G2 DNA damage checkpoint; G2/M transition of mitotic cell cycle; inner cell mass cell proliferation; mitotic cell cycle checkpoint; negative regulation of DNA biosynthetic process; negative regulation of G2/M transition of mitotic cell cycle; negative regulation of mitosis; nucleus organization; peptidyl-threonine phosphorylation; phosphorylation; positive regulation of cell cycle; protein amino acid phosphorylation; regulation of cell proliferation; regulation of double-strand break repair via homologous recombination; regulation of gene expression; regulation of histone H3-K9 acetylation; regulation of mitotic centrosome separation; regulation of transcription from RNA polymerase II promoter in response to UV-induced DNA damage
Research Articles on CHEK1
Similar Products
Product Notes
The CHEK1 chek1 (Catalog #AAA2017265) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Checkpoint Homolog (CHEK1) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Checkpoint Homolog (CHEK1) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the CHEK1 chek1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- CGIVLTA MLAGELPWDQ PSDSCQEYSD WKEKKTYLNP WKKIDSAPLA LLHKILVETP SARITIPDIK KDRWYNKPLN RGAKRPRATS GGMSESSSGF SKHIHSNLDF SPVNNGSSEE TVKFSSSQPE PRTGLSLWDT GPSNVDKLVQ GISFSQPTCP EHMLVNSQLL GTPGSSQNPW QRLVKRMTRF FTKLDADKSY. It is sometimes possible for the material contained within the vial of "Checkpoint Homolog (CHEK1), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
