CHK1 / CHEK1 recombinant protein

Recombinant Mouse CHK1 / CHEK1 Protein (His & GST tag)

Cat. Num. AAA2546612

• Gene Names: Chek1; Chk1; rad27; C85740
• Purity: > 90 % as determined by SDS-PAGE
• Synonyms: CHK1 / CHEK1; Recombinant Mouse CHK1 / CHEK1 Protein (His & GST tag); CHEK1; Chk1; CHK1 / CHEK1 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: > 90 % as determined by SDS-PAGE
• Form/Format: Supplied as sterile 20mM Tris, 500mM NaCl, pH 8.5, 3mM DTT, 10% gly
• Sequence Length: 476

• Application Notes: The recombinant mouse CHEK1/GST chimera consists of 713 amino acids and has a calculated molecular mass of 82.2 kDa. The recombinant protein migrates as an approximately 78 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for CHK1 / CHEK1 recombinant protein

Background: CHK1 / CHEK1 contains 1 protein kinase domain and belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family, NIM1 subfamily. It is a member of checkpoint kinases (Chks). Chks Checkpoint kinases (Chks) are serine/threonine kinases that are involved in the control of the cell cycle. There are two subtype s of chks that have so far been identified, CHK1 / CHEK1 and Chk2. They are essential components to delay cell cycle progression in normal and damaged cells and can act at all three cell cycle checkpoints. Chks are activated by phosphorylation. ATR kinase phosphorylates CHK1 / CHEK1 in response to single strand DNA breaks and ATM kinase phosphorylates Chk2 in response to double strand breaks. Chks phosphorylate Cdc25 phosphatase at Ser216, which leads to Cdc25 sequestration in the cytoplasm. Chks have a role in the physiological stress of hypoxia/reoxygenation. CHK1 / CHEK1 is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. CHK1 / CHEK1 may also negatively regulate cell cycle progression during unperturbed cell cycles.

Description: A DNA sequence encoding the mouse CHEK1 (O35280-1) (Met1-Thr476) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 31542385
• NCBI GeneID: 12649
• NCBI Accession #: NP_031717.2
• NCBI GenBank Nucleotide #: NM_007691.5
• UniProt Accession #: O35280; O54925; Q8CI40; Q9D0N2
• Molecular Weight: 43,627 Da
• NCBI Official Full Name: serine/threonine-protein kinase Chk1
• NCBI Official Synonym Full Names: checkpoint kinase 1
• NCBI Official Symbol: Chek1
• NCBI Official Synonym Symbols: Chk1; rad27; C85740
• NCBI Protein Information: serine/threonine-protein kinase Chk1
• UniProt Protein Name: Serine/threonine-protein kinase Chk1
• UniProt Gene Name: Chek1
• UniProt Synonym Gene Names: Chk1

Uniprot Description

Chk1: a protein kinase of the CAMKL family. Required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. Plays an important role in both S and G2 checkpoints, embryonic development and tumor suppression. Phosphorylated and activated by ATM/ATR following DNA damage, it binds to single-stranded DNA and DNA ends and has a role in the repair of double strand breaks. Activated Chk1 can phosphorylate and inactivate cdc25C via phosphorylation and 14-3-3 binding, blocking the activation of cdc2 and transition into M-phase. Other substrates include p53. Implicated in resistance to apoptosis in response to chemotherapy. Inhibitors under development to chemosensitize tumors. Somatic mutations found in stomach tumors, and in colon and endometrial tumors, where CHK1 may be a target of microsatellite instability. Inhibitors: SB218078, UNC-01.

Protein type: CAMK group; CAMKL family; CHK1 subfamily; EC 2.7.11.1; Kinase, protein; Protein kinase, CAMK; Protein kinase, Ser/Thr (non-receptor); Tumor suppressor

Chromosomal Location of Human Ortholog: 9|9 A4

Cellular Component: centrosome; chromatin; chromosome, telomeric region; condensed nuclear chromosome; extracellular space; intracellular membrane-bound organelle; nucleoplasm; nucleus; replication fork

Molecular Function: protein binding; protein kinase activity; protein serine/threonine kinase activity

Biological Process: chromatin-mediated maintenance of transcription; DNA damage checkpoint; DNA damage induced protein phosphorylation; DNA repair; G2/M transition DNA damage checkpoint; G2/M transition of mitotic cell cycle; inner cell mass cell proliferation; mitotic cell cycle checkpoint; negative regulation of mitosis; nuclear organization and biogenesis; peptidyl-threonine phosphorylation; protein amino acid phosphorylation; regulation of cell proliferation; regulation of gene expression; regulation of mitotic centrosome separation; response to DNA damage stimulus