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Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Rabbit anti-Mouse Non Metastatic Cells 2, Protein NM23B Expressed In (NME2) Polyclonal Antibody | anti-NME2 antibody

Polyclonal Antibody to Non Metastatic Cells 2, Protein NM23B Expressed In (NME2)

Gene Names
Nme2; NM23B; NM23-H2; nm23-M2
Reactivity
Mouse
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Non Metastatic Cells 2; Protein NM23B Expressed In (NME2); Polyclonal Antibody; Polyclonal Antibody to Non Metastatic Cells 2; anti-NME2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against NME2. It has been selected for its ability to recognize NME2 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-FIA IKPDGVQRGL VGEIIKRFEQ KGFRLVAMKF LRASEEHLKQ HYIDLKDRPF FPGLVKYMNS GPVVAMVWEG LNVVKTGRVM LGETNPADSK PGTIRGDFCI QVGRNIIHGS DSVESAEKEI HLWFKPEELI DYKSCAHDW
Applicable Applications for anti-NME2 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant NME2 (Phe8~Trp149) expressed in E.coli.
Cross Reactivity
Mouse
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2081776
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DABstainingonIHC-P.Samples:MouseTissue))

Immunohistochemistry (IHC) (DABstainingonIHC-P.Samples:MouseTissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
17,363 Da
NCBI Official Full Name
nucleoside diphosphate kinase B
NCBI Official Synonym Full Names
NME/NM23 nucleoside diphosphate kinase 2
NCBI Official Symbol
Nme2
NCBI Official Synonym Symbols
NM23B; NM23-H2; nm23-M2
NCBI Protein Information
nucleoside diphosphate kinase B
UniProt Protein Name
Nucleoside diphosphate kinase B
UniProt Gene Name
Nme2
UniProt Synonym Gene Names
NDK B; NDP kinase B

Uniprot Description

NME2: Major role in the synthesis of nucleoside triphosphates other than ATP. Negatively regulates Rho activity by interacting with AKAP13/LBC. Acts as a transcriptional activator of the MYC gene; binds DNA non-specifically (PubMed:8392752). Exhibits histidine protein kinase activity. Belongs to the NDK family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.13.3; EC 2.7.4.6; Kinase, nucleoside diphosphate; Kinase, other; NDK family; Nuclear receptor co-regulator; Nucleotide Metabolism - purine; Nucleotide Metabolism - pyrimidine; Other group

Chromosomal Location of Human Ortholog: 11|11 D

Cellular Component: cell periphery; cell projection; cytoplasm; cytosol; extracellular exosome; intermediate filament; lamellipodium; mitochondrial membrane; mitochondrion; myelin sheath; nucleus; perinuclear region of cytoplasm; ruffle

Molecular Function: ATP binding; drug binding; enzyme binding; fatty acid binding; intermediate filament binding; kinase activity; metal ion binding; nucleoside diphosphate kinase activity; nucleotide binding; protein binding; protein histidine kinase activity; protein serine/threonine kinase activity; transferase activity

Biological Process: CTP biosynthetic process; GTP biosynthetic process; integrin-mediated signaling pathway; negative regulation of apoptosis; negative regulation of myeloid leukocyte differentiation; nucleoside diphosphate phosphorylation; nucleoside triphosphate biosynthetic process; nucleotide metabolic process; phosphorylation; positive regulation of cAMP biosynthetic process; positive regulation of epithelial cell proliferation; positive regulation of keratinocyte differentiation; positive regulation of neuron projection development; positive regulation of transcription from RNA polymerase II promoter; protein autophosphorylation; protein oligomerization; regulation of apoptosis; regulation of epidermis development; UTP biosynthetic process

Research Articles on NME2

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Product Notes

The NME2 nme2 (Catalog #AAA2006253) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Non Metastatic Cells 2, Protein NM23B Expressed In (NME2) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Non Metastatic Cells 2, Protein NM23B Expressed In (NME2) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the NME2 nme2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-FIA IKPDGVQRGL VGEIIKRFEQ KGFRLVAMKF LRASEEHLKQ HYIDLKDRPF FPGLVKYMNS GPVVAMVWEG LNVVKTGRVM LGETNPADSK PGTIRGDFCI QVGRNIIHGS DSVESAEKEI HLWFKPEELI DYKSCAHDW. It is sometimes possible for the material contained within the vial of "Non Metastatic Cells 2, Protein NM23B Expressed In (NME2), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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