Rabbit CENPW Polyclonal Antibody | anti-CENPW antibody

CENPW Antibody

Cat. Num. AAA153704

• Gene Names: CENPW; CUG2; CENP-W; C6orf173
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: CENPW Antibody is affinity chromatography purified via peptide column.
• Synonyms: CENPW; Polyclonal Antibody; CENPW Antibody; CUG2; CENP-W; C6orf173; Centromere protein W; Cancer-up-regulated gene 2 protein; centromere protein W; anti-CENPW antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: At least three isoforms of CENPW are known to exist; this antibody will detect all three isoforms.
• Purity/Purification: CENPW Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 88

• Applicable Applications for anti-CENPW antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: CENPW antibody can be used for detection of CENPW by Western blot at 0.5 - 1 mug/mL.
• Conjugate: Unconjugated
• Immunogen: CENPW antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human CENPW.
• Buffer: CENPW Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: CENPW antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of CENPW in HeLa cell lysate lysate with CENPW antibody at (A) 0.5 and (B) 1 μg/mL.)

Related Product Information for anti-CENPW antibody

CENPW Antibody: CENPW was initially identified as a gene that was upregulated in multiple cancers, and whose overexpression in mouse fibroblast cells gave rise to distinct cancer-specific phenotypes. It was later found to be a nuclear protein that associated with CENPT, a component of CENPA nucleosome complex in the centromere, and is required for proper kinetochore function. CENPW also specifically interacts with the nucleolar phosphoprotein nucleophosmin, also known as B23 It has been suggested that nucleophosmin functions in the assembly of the kinetochore by interacting with CENPW during interphase. Overexpression of CENPW in the SKOV-3 human ovarian cancer cell line as well as in the zebrafish embryo led to apoptosis, suggesting that high levels of CENPW induces apoptotic cell death.

NCBI and Uniprot Product Information

• NCBI GI #: 60302883
• NCBI GeneID: 387103
• NCBI Accession #: NP_001012525
• NCBI GenBank Nucleotide #: NM_001012507.3
• UniProt Accession #: Q5EE01; A6NIR0; A6NJC2
• Molecular Weight: 11,835 Da
• NCBI Official Full Name: centromere protein W isoform b
• NCBI Official Synonym Full Names: centromere protein W
• NCBI Official Symbol: CENPW
• NCBI Official Synonym Symbols: CUG2; CENP-W; C6orf173
• NCBI Protein Information: centromere protein W; cancer-upregulated gene 2; cancer-up-regulated gene 2 protein
• UniProt Protein Name: Centromere protein W
• Protein Family: Centromere protein
• UniProt Gene Name: CENPW
• UniProt Synonym Gene Names: C6orf173; CUG2; CENP-W
• UniProt Entry Name: CENPW_HUMAN

Uniprot Description

CENPW: Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Part of a nucleosome-associated complex that binds specifically to histone H3-containing nucleosomes at the centromere, as opposed to nucleosomes containing CENPA. Component of the heterotetrameric CENP-T-W-S-X complex that binds and supercoils DNA, and plays an important role in kinetochore assembly. CENPW has a fundamental role in kinetochore assembly and function. It is one of the inner kinetochore proteins, with most further proteins binding downstream. Required for normal chromosome organization and normal progress through mitosis. Belongs to the CENPW family.

Protein type: Nucleolus

Chromosomal Location of Human Ortholog: 6q22.32

Cellular Component: kinetochore; nucleoplasm; nuclear matrix; nucleolus; chromosome, pericentric region

Molecular Function: protein binding; DNA binding; protein heterodimerization activity

Biological Process: nucleosome assembly; mitosis; kinetochore assembly; DNA replication-independent nucleosome assembly at centromere; cell division; mitotic cell cycle; chromosome organization and biogenesis; chromosome segregation