Rabbit GNB1 Polyclonal Antibody | anti-GNB1 antibody

GNB1 Antibody (N-term)

Cat. Num. AAA9212681

• Reactivity: Human (Predicted Reactivity: Bovine, Hamster, Zebrafish, Mouse, Rat, Xenopus)
• Applications: Western Blot, ELISA, Flow Cytometry, Functional Assay
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: GNB1; Polyclonal Antibody; GNB1 Antibody (N-term); Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1; Transducin beta chain 1; anti-GNB1 antibody

• Host: Rabbit
• Reactivity: Human (Predicted Reactivity: Bovine, Hamster, Zebrafish, Mouse, Rat, Xenopus)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This GNB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human GNB1.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Positions: 1-30
• Sequence Length: 340

• Applicable Applications for anti-GNB1 antibody: Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS)
• Application Notes: WB~~1:1000; FC~~1:10~50
• Antigen Type: Synthetic Peptide
• Clone Names: RB26119
• Antigen Source: HUMAN
• Function: Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein- effector interaction.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-GNB1 Antibody (N-term) at 1:1000 dilution Lane 1: human brain lysate Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 37 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

Flow Cytometry (FC/FACS)

(GNB1 Antibody (N-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-GNB1 antibody

GNB1 integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. This protein uses alternative polyadenylation signals.

Product Categories/Family for anti-GNB1 antibody

Cancer; Metabolism; Signal Transduction

References

Ahmed, S.M., et al. J. Biol. Chem. 285(9):6538-6551(2010)
Gutman, O., et al. J. Biol. Chem. 285(6):3905-3915(2010)
Knezevic, N., et al. J. Exp. Med. 206(12):2761-2777(2009)

NCBI and Uniprot Product Information

• NCBI GI #: 11321585
• NCBI GeneID: 2782
• NCBI Accession #: NP_002065.1
• NCBI GenBank Nucleotide #: NM_002074.4
• UniProt Accession #: P62873; P04697; P04901; Q1RMY8; B1AJZ7
• Molecular Weight: 37377
• NCBI Official Full Name: guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 isoform 1
• NCBI Official Synonym Full Names: guanine nucleotide binding protein (G protein), beta polypeptide 1
• NCBI Official Symbol: GNB1
• NCBI Protein Information: guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
• UniProt Protein Name: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
• UniProt Gene Name: GNB1
• UniProt Entry Name: GBB1_HUMAN

NCBI Description

Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]

Uniprot Description

G-beta 1: Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein- effector interaction. Belongs to the WD repeat G protein beta family.

Protein type: G protein, heterotrimeric; G protein, heterotrimeric beta; G protein

Chromosomal Location of Human Ortholog: 1p36.33

Cellular Component: photoreceptor inner segment; membrane; lysosomal membrane; dendrite; plasma membrane; heterotrimeric G-protein complex; intracellular; vesicle

Molecular Function: GTPase activity; signal transducer activity; GTPase binding; protein binding; spectrin binding; protein complex binding; type 1 angiotensin receptor binding

Biological Process: platelet activation; phototransduction, visible light; acetylcholine receptor signaling, muscarinic pathway; sensory perception of taste; signal transduction; rhodopsin mediated signaling; cell proliferation; synaptic transmission; elevation of cytosolic calcium ion concentration; regulation of rhodopsin mediated signaling; retina development in camera-type eye; Ras protein signal transduction; energy reserve metabolic process; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); blood coagulation; dopamine receptor, adenylate cyclase activating pathway