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Western Blot (WB) (Western blot analysis of TSPY1L in A20 cell lysate with TSPY1L antibody at (A) 0.5 and (B) 1 μg/mL)

Rabbit anti-Human, Mouse TSPY1L Polyclonal Antibody | anti-TSPY1 antibody

TSPY1L Antibody

Gene Names
TSPY1; CT78; TSPY; DYS14; pJA923
Reactivity
Human, Mouse
Applications
ELISA, Western Blot
Purity
TSPY1L Antibody is affinity chromatography purified via peptide column.
Synonyms
TSPY1L; Polyclonal Antibody; TSPY1L Antibody; CT78; TSPY; DYS14; pJA923; Testis-specific Y-encoded protein 1; testis specific protein; Y-linked 1; anti-TSPY1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
TSPY1L antibody is human and mouse reactive. At least three isoforms of TSPY1 are known to exist; this antibody will detect only TSPY1L.
Purity/Purification
TSPY1L Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
308
Applicable Applications for anti-TSPY1 antibody
ELISA (EIA), Western Blot (WB)
Application Notes
TSPY1L antibody can be used for detection of TSPY1L by Western blot at 0.5 - 1 mug/mL.
Conjugate
Unconjugated
Immunogen
Rabbit polyclonal TSPY1L antibody was raised against a 20 amino acid peptide near the carboxy terminus of human TSPY1L.
Buffer
TSPY1L Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
TSPY1L antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of TSPY1L in A20 cell lysate with TSPY1L antibody at (A) 0.5 and (B) 1 μg/mL)

Western Blot (WB) (Western blot analysis of TSPY1L in A20 cell lysate with TSPY1L antibody at (A) 0.5 and (B) 1 μg/mL)
Related Product Information for anti-TSPY1 antibody
TSPY1L Antibody: Testis-specific protein on Y chromosome (TSPY1) is an ampliconic gene on the Y chromosome that has been associated with gonadoblastoma. Recent experiments have shown that in androgen-dependent testicular germ-cell tumors, TSPY1 can repress the androgen-bound androgen receptor (AR), a member of the nuclear steroid hormone receptor family that acts as a ligand-inducible transcription factor, suggesting that TSPY1 is a repressor of cell proliferation in germ-cell tumors and potentially in normal gonadal cells during early development. Two distinct isoforms of TSPY1, TSPY1L and TSPY1S, are known to exist.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
34 kDa
NCBI Official Full Name
testis-specific Y-encoded protein 1 isoform TSPY-L
NCBI Official Synonym Full Names
testis specific protein, Y-linked 1
NCBI Official Symbol
TSPY1
NCBI Official Synonym Symbols
CT78; TSPY; DYS14; pJA923
NCBI Protein Information
testis-specific Y-encoded protein 1; cancer/testis antigen 78
UniProt Protein Name
Testis-specific Y-encoded protein 1
UniProt Gene Name
TSPY1
UniProt Synonym Gene Names
TSPY; CT78
UniProt Entry Name
TSPY1_HUMAN

NCBI Description

The protein encoded by this gene is found only in testicular tissue and may be involved in spermatogenesis. Approximately 35 copies of this gene are present in humans, but only a single, nonfunctional orthologous gene is found in mouse. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2010]

Uniprot Description

TSPY1: May be involved in sperm differentiation and proliferation. TSPY is located in the gonadoblastoma critical region and is preferentially expressed in tumor germ cells of gonadoblastoma specimens. Expression also correlates with testicular seminoma and tumorigenesis of the prostate gland. Belongs to the nucleosome assembly protein (NAP) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cancer Testis Antigen (CTA)

Chromosomal Location of Human Ortholog: Yp11.2

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding

Biological Process: cell proliferation; nucleosome assembly; gonadal mesoderm development; spermatogenesis; cell differentiation; sex differentiation

Disease: Gonadoblastoma

Research Articles on TSPY1

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Product Notes

The TSPY1 tspy1 (Catalog #AAA153456) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TSPY1L Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TSPY1L can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). TSPY1L antibody can be used for detection of TSPY1L by Western blot at 0.5 - 1 mug/mL. Researchers should empirically determine the suitability of the TSPY1 tspy1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TSPY1L, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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