Rabbit RILPL1 Polyclonal Antibody | anti-RILPL1 antibody

RILPL1 Antibody

Cat. Num. AAA150318

• Gene Names: RILPL1; RLP1; GOSPEL
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunofluorescence
• Purity: RILPL1 Antibody is affinity chromatography purified via peptide column.
• Synonyms: RILPL1; Polyclonal Antibody; RILPL1 Antibody; RLP1; GOSPEL; RILP-like protein 1; Rab-interacting lysosomal-like protein 1; Rab interacting lysosomal protein-like 1; anti-RILPL1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: At least three isoforms of RILPL1 are known to exist; this antibody will detect the two shorter isoforms. RILPL1 antibody is predicted to not cross-react with RILP.
• Purity/Purification: RILPL1 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 403

• Applicable Applications for anti-RILPL1 antibody: ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
• Application Notes: RILPL1 antibody can be used for detection of RILPL1 by Western blot at 0.5 and 1 mug/mL. Antibody can also be used for immunofluorescence starting at 20 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: RILPL1 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human RILPL1.
• Buffer: RILPL1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: RILPL1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of RILPL1 in rat cerebellum tissue lysate with RILPL1 antibody at (A) 0.5 and (B) 1 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of RILPL1 in mouse brain cells with RILPL1 antibody at 20 μg/mL.)

Related Product Information for anti-RILPL1 antibody

RILPL1 Antibody: While the function of the Rab interacting lysosomal protein-like 1 (RILPL1) remains unknown, the homologous protein RILP is a small GTPase that controls transport to endocytic compartments, suggesting RILPL1 may have similar functions. However, overexpression of RILP caused enlarged lysosomes that are more centrally located in the cell, while overexpression of RILPL1 had no visible effect on the location or morphology of lysosomes.

NCBI and Uniprot Product Information

• NCBI GI #: 195947382
• NCBI GeneID: 353116
• NCBI Accession #: AAH89444
• NCBI GenBank Nucleotide #: NM_178314.3
• UniProt Accession #: Q5EBL4; Q66K36; Q8N1M0
• Molecular Weight: 29,468 Da
• NCBI Official Full Name: RILP-like protein 1
• NCBI Official Synonym Full Names: Rab interacting lysosomal protein-like 1
• NCBI Official Symbol: RILPL1
• NCBI Official Synonym Symbols: RLP1; GOSPEL
• NCBI Protein Information: RILP-like protein 1; rab-interacting lysosomal-like protein 1
• UniProt Protein Name: RILP-like protein 1
• Protein Family: RILP-like protein
• UniProt Gene Name: RILPL1
• UniProt Synonym Gene Names: RLP1
• UniProt Entry Name: RIPL1_HUMAN

Uniprot Description

RILPL1: Neuroprotective protein, which acts by sequestring GAPDH in the cytosol and prevent the apoptotic function of GAPDH in the nucleus. Competes with SIAH1 for binding GAPDH. Does not regulate lysosomal morphology and distribution. Belongs to the RILPL family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 12q24.31

Cellular Component: nucleoplasm; centrosome; cytoplasm; plasma membrane; cytosol

Biological Process: intracellular protein transport