Rabbit CD194 Polyclonal Antibody | anti-CD194 antibody
RABBIT F(ab')2 ANTI MOUSE CD194
Gene Names
Ccr4; LESTR; Sdf1r; CHEMR1; Cmkbr4; C-C CKR-4
Applications
Immunohistochemistry, ELISA, Flow Cytometry, Functional Assay, Western Blot
Synonyms
CD194; Polyclonal Antibody; RABBIT F(ab')2 ANTI MOUSE CD194; anti-CD194 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Form/Format
Purified
F(ab')2 fragment of purified IgG - liquid
F(ab')2 fragment of purified IgG - liquid
Sequence Length
360
Applicable Applications for anti-CD194 antibody
Immunohistology Frozen, ELISA (EIA), Flow cytometry (FC/FACS), Western Blot (WB)
Application Notes
ELISA: This product is suitable for use in indirect ELISA applications.
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Immunohistology - Frozen: Maximum Dilution: 1/300
ELISA: Maximum Dilution: 1/50000
Flow Cytometry: Maximum Dilution: 1/20
Western Blotting: Maximum Dilution: 1/1000
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Immunohistology - Frozen: Maximum Dilution: 1/300
ELISA: Maximum Dilution: 1/50000
Flow Cytometry: Maximum Dilution: 1/20
Western Blotting: Maximum Dilution: 1/1000
Perservative Stabilisers
0.1% Sodium Azide (NaN3)
0.1% Bovine Serum Albumin
0.1% Bovine Serum Albumin
Buffer Solution
Phosphate buffered saline
Antiserum Preparation
Antiserum to mouse CD194 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Target Species
Mouse
Preparation and Storage
Store at -20 degree C only. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Related Product Information for anti-CD194 antibody
Rabbit anti mouse CD194 antibody recognizes mouse CD194, otherwise known as CCR4, C-C chemokine receptor type 4, C-C CKR-4or Cmkbr4. CD194 is a 360 amino acid ~42kDa transmembrane glycoprotein and member of the G-protein coupled receptor 1 family, preferentially expressed by CD4+ Th2 (type 2 helper T) cells, which acts as a high affinity receptor for the chemokines MDC (macrophage-derived chemokine), MCP-1, MIP-1, RANTES and TARC. Rabbit anti mouse CD194 polyclonal antibody is reported as suitable for use in immunocytochemistry on human peripheral blood leucocytes or Jurkat cells.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
41,408 Da
NCBI Official Full Name
C-C chemokine receptor type 4
NCBI Official Synonym Full Names
chemokine (C-C motif) receptor 4
NCBI Official Symbol
Ccr4
NCBI Official Synonym Symbols
LESTR; Sdf1r; CHEMR1; Cmkbr4; C-C CKR-4
NCBI Protein Information
C-C chemokine receptor type 4; chemokine (C-C) receptor 4; fusin; leukocyte-expressed seven-transmembrane-domain receptor
UniProt Protein Name
C-C chemokine receptor type 4
UniProt Gene Name
Ccr4
UniProt Synonym Gene Names
Cmkbr4; C-C CKR-4; CC-CKR-4; CCR-4; CCR4
UniProt Entry Name
CCR4_MOUSE
Uniprot Description
CCR4: High affinity receptor for the C-C type chemokines CCL17/TARC and CCL22/MDC. The activity of this receptor is mediated by G(i) proteins which activate a phosphatidylinositol- calcium second messenger system. Can function as a chemoattractant homing receptor on circulating memory lymphocytes and as a coreceptor for some primary HIV-2 isolates. In the CNS, could mediate hippocampal-neuron survival. Belongs to the G-protein coupled receptor 1 family.
Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; GPCR, family 1; Membrane protein, multi-pass; Receptor, GPCR
Cellular Component: membrane; cell soma; plasma membrane; integral to membrane; external side of plasma membrane
Molecular Function: G-protein coupled receptor activity; signal transducer activity; C-C chemokine receptor activity; chemokine receptor activity
Biological Process: G-protein coupled receptor protein signaling pathway; positive regulation of positive chemotaxis; neuron migration; immune response; chemotaxis; inflammatory response; signal transduction; tolerance induction
Research Articles on CD194
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Product Notes
The CD194 ccr4 (Catalog #AAA223660) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD194 can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen, ELISA (EIA), Flow cytometry (FC/FACS), Western Blot (WB). ELISA: This product is suitable for use in indirect ELISA applications. Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. Immunohistology - Frozen: Maximum Dilution: 1/300 ELISA: Maximum Dilution: 1/50000 Flow Cytometry: Maximum Dilution: 1/20 Western Blotting: Maximum Dilution: 1/1000. Researchers should empirically determine the suitability of the CD194 ccr4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD194, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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