Goat CCR10 Polyclonal Antibody | anti-CCR10 antibody

GOAT ANTI HUMAN CCR10

Cat. Num. AAA223798

• Gene Names: CCR10; GPR2
• Applications: ELISA, Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Synonyms: CCR10; Polyclonal Antibody; GOAT ANTI HUMAN CCR10; anti-CCR10 antibody

• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 362

• Applicable Applications for anti-CCR10 antibody: ELISA (EIA), Flow cytometry (FC/FACS), Immunohistology Paraffin, Western Blot (WB)
• Application Notes: ELISA: This product is suitable for use in indirect ELISA applications.; Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. ; ELISA: Maximum Dilution: 1/50,000; Flow Cytometry: Maximum Dilution: Neat; Immunohistology - Paraffin: Maximum Dilution: 1/125; Western Blotting: Maximum Dilution: 1/30,000
• Perservative Stabilisers: 0.1% Sodium Azide; 0.1% Bovine Serum Albumin
• Histology Positive Control Tissue: Spleen or tonsil; Buffer Solution
• Antiserum Preparation: Antiserum to human CCR10 was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Paraffin embedded human spleen stained with Goat anti Human CCR10 (MBS223798))

Testing Data #2

(Paraffin embedded human tonsil stained with Goat anti Human CCR10 (MBS223798))

Related Product Information for anti-CCR10 antibody

Goat anti Human CCR10 antibody recognizes human CCR10 (C-C chemokine receptor type 10), otherwise known as GPR2, (G-protein coupled receptor 2), a transmembrane glycoprotein and member of the G-protein coupled receptor 1 family, expressed by melanocytes, dermal fibroblasts, and dermal microvascular endothelial cells, which acts as a receptor for the skin-associated chemokine CCL27 (SCYA27/CTACK), and is implicated in T cell-mediated skin inflammation and possibly skin homeostasis.

NCBI and Uniprot Product Information

• NCBI GI #: 156104886
• NCBI GeneID: 2826
• NCBI Accession #: NP_057686.2
• NCBI GenBank Nucleotide #: NM_016602.2
• UniProt Accession #: P46092; Q4V749; Q6T7X2; Q9NZG2
• Molecular Weight: 38,416 Da
• NCBI Official Full Name: C-C chemokine receptor type 10
• NCBI Official Synonym Full Names: chemokine (C-C motif) receptor 10
• NCBI Official Symbol: CCR10
• NCBI Official Synonym Symbols: GPR2
• NCBI Protein Information: C-C chemokine receptor type 10; C-C CKR-10; CC chemokine receptor 10; CC-CKR-10; CCR-10; G protein-coupled receptor 2; G-protein coupled receptor 2
• UniProt Protein Name: C-C chemokine receptor type 10
• Protein Family: C-C chemokine receptor
• UniProt Gene Name: CCR10
• UniProt Synonym Gene Names: GPR2; C-C CKR-10; CC-CKR-10; CCR-10
• UniProt Entry Name: CCR10_HUMAN

NCBI Description

Chemokines are a group of small (approximately 8 to 14 kD), mostly basic, structurally related molecules that regulate cell trafficking of various types of leukocytes through interactions with a subset of 7-transmembrane, G protein-coupled receptors. Chemokines also play fundamental roles in the development, homeostasis, and function of the immune system, and they have effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis. Chemokines are divided into 2 major subfamilies, CXC and CC, based on the arrangement of the first 2 of the 4 conserved cysteine residues; the 2 cysteines are separated by a single amino acid in CXC chemokines and are adjacent in CC chemokines. CCR10 is the receptor for CCL27 (SCYA27; MIM 604833); CCR10-CCL27 interactions are involved in T cell-mediated skin inflammation (Homey et al., 2002 [PubMed 11821900]).[supplied by OMIM, Mar 2008]

Uniprot Description

CCR10: Receptor for chemokines SCYA27 and SCYA28. Subsequently transduces a signal by increasing the intracellular calcium ions level and stimulates chemotaxis in a pre-B cell line. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, integral; GPCR, family 1; Receptor, GPCR; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 17q21.1-q21.3

Cellular Component: cell surface; integral to plasma membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; C-C chemokine receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; elevation of cytosolic calcium ion concentration; immune response; chemotaxis