Western Blot/Blotting
(Western blot analysis of caspase-4 in human spleen cells with caspase-4 antibody at (A) 1 and (B) 2 g/ml)
Rabbit anti-Human Caspase-4 Precursor Polyclonal Antibody | anti-CASP4 antibody
Caspase-4 Precursor (IN)
Western Blot/Blotting
(Western blot analysis of caspase-4 in human spleen cells with caspase-4 antibody at (A) 1 and (B) 2 g/ml)
Western Blot/Blotting
(Western blot analysis of caspase-4 in human spleen cells with caspase-4 antibody at (A) 1 and (B) 2 g/ml)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein that is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain and a large and small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This caspase is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. When overexpressed, this gene induces cell apoptosis. Alternative splicing results in transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]
Uniprot Description
Function: Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves caspase-1. Ref.11
Catalytic activity: Strict requirement for Asp at the P1 position. It has a preferred cleavage sequence of Tyr-Val-Ala-Asp-|- but also cleaves at Asp-Glu-Val-Asp-|-. Ref.11
Enzyme regulation: Inhibited by the effector protein NleF that is produced by pathogenic E.coli; this inhibits apoptosis. Ref.11
Subunit structure: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a small and a large subunit
By similarity. Interacts with NleF from pathogenic E.coli. Ref.11
Tissue specificity: Widely expressed, with highest levels in spleen and lung. Moderate expression in heart and liver, low expression in skeletal muscle, kidney and testis. Not found in the brain.
Post-translational modification: The two subunits are derived from the precursor sequence by an autocatalytic mechanism or by cleavage by Caspase-8.
Sequence similarities: Belongs to the peptidase C14A family.Contains 1 CARD domain.
Research Articles on CASP4
Similar Products
Product Notes
The CASP4 casp4 (Catalog #AAA395812) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Caspase-4 Precursor (IN) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Caspase-4 Precursor can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Researchers should empirically determine the suitability of the CASP4 casp4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Caspase-4 Precursor, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
