Rabbit anti-Human CASP4 / Caspase 4 Polyclonal Antibody | anti-CASP4 antibody

Rabbit Polyclonal to Human CASP4 / Caspase 4

Cat. Num. AAA247811

• Gene Names: CASP4; TX; ICH-2; Mih1/TX; ICEREL-II; ICE(rel)II
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Immunoaffinity Purified
• Synonyms: CASP4 / Caspase 4; Polyclonal Antibody; Rabbit Polyclonal to Human CASP4 / Caspase 4; Anti-CASP4 / Caspase 4 Antibody (Internal) IHC-plus; CASP4; CASP-4; Casp11; Caspase 4; Caspase-4; Cysteine protease icerel-ii; ICE(rel)-II; ICE(rel)II; ICH-2; Ich-3; ICH2; Protease ICH-2; TX; Tx protease; Caspase 11; ICEREL-II; Mih1/TX; Protease TX; Human CASP4; anti-CASP4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: 15 amino acid peptide from near the center of human Caspase-4
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.02% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 377

• Applicable Applications for anti-CASP4 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
• Application Notes: IHC-P (5 ug/ml), WB (2 ug/ml)
• Target Species: Human
• Immunogen Description: Synthetic peptide from human CASP4 / Caspase 4.
• Immunogen Type: Synthetic peptide
• Immunogen: CASP4 / Caspase 4 antibody was raised against synthetic peptide from human CASP4 / Caspase 4.
• Antigen Modification: Internal
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.

Immunohistochemistry (IHC)

(Anti-CASP4 / Caspase 4 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-CASP4 / Caspase 4 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of Caspase-4 in A20 cells with Caspase-4 antibody at 10 ug/ml.)

Western Blot (WB)

(Western blot of caspase-4 in human spleen cells with caspase-4 antibody at (A) 1 and (B) 2 ug/ml.)

Related Product Information for anti-CASP4 antibody

Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. Members of this subfamily include caspase-1, -4, -5, and -12. Activation of these caspases results in the cleavage and activation of proinflammatory cytokines such as IL-1b and IL-18.

Product Categories/Family for anti-CASP4 antibody

Family: Protease; Subfamily: Cysteine C14

NCBI and Uniprot Product Information

• NCBI GI #: 4502577
• NCBI GeneID: 837
• NCBI Accession #: NP_001216.1
• NCBI GenBank Nucleotide #: NM_001225.3
• UniProt Accession #: P49662; A2NHL8; A2NHM0
• Molecular Weight: 36,705 Da
• NCBI Official Full Name: caspase-4 isoform alpha
• NCBI Official Synonym Full Names: caspase 4, apoptosis-related cysteine peptidase
• NCBI Official Symbol: CASP4
• NCBI Official Synonym Symbols: TX; ICH-2; Mih1/TX; ICEREL-II; ICE(rel)II
• NCBI Protein Information: caspase-4; CASP-4; ICE(rel)-II; protease TX; protease ICH-2; apoptotic cysteine protease Mih1/TX; caspase 4, apoptosis-related cysteine protease
• UniProt Protein Name: Caspase-4
• Protein Family: Caspase
• UniProt Gene Name: CASP4
• UniProt Synonym Gene Names: ICH2; CASP-4
• UniProt Entry Name: CASP4_HUMAN

NCBI Description

This gene encodes a protein that is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain and a large and small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This caspase is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. When overexpressed, this gene induces cell apoptosis. Alternative splicing results in transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

CASP4: Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves caspase-1. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a small and a large subunit. Widely expressed, with highest levels in spleen and lung. Moderate expression in heart and liver, low expression in skeletal muscle, kidney and testis. Not found in the brain. Belongs to the peptidase C14A family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.22.64; Protease; Apoptosis

Chromosomal Location of Human Ortholog: 11q22.2-q22.3

Cellular Component: endoplasmic reticulum membrane; mitochondrion; cytoplasm

Molecular Function: cysteine-type endopeptidase activity

Biological Process: regulation of apoptosis; apoptosis; regulation of inflammatory response; proteolysis