Rabbit anti-Human, Mouse Caspase-4 Polyclonal Antibody | anti-CASP4 antibody

Caspase-4 Antibody

Cat. Num. AAA150271

• Gene Names: CASP4; TX; ICH-2; Mih1/TX; ICEREL-II; ICE(rel)II
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Caspase-4 Antibody is affinity chromatography purified via peptide column.
• Synonyms: Caspase-4; Polyclonal Antibody; Caspase-4 Antibody; TX; ICH-2; Mih1/TX; ICEREL-II; ICE(rel)II; ICH2; Protease ICH-2; CASP-4; caspase 4; apoptosis-related cysteine peptidase; anti-CASP4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Depending on cell lines or tissues used, other cleavage products may be observed.
• Purity/Purification: Caspase-4 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 377

• Applicable Applications for anti-CASP4 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Casp-4 antibody can be used for the detection of Caspase-4 by Western blot at 0.5 - 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 2 mug/mL. For immunofluorescence start at 10 mug/mL.
• Conjugate: Unconjugated
• Immunogen: Caspase-4 antibody was raised against a 16 amino acid synthetic peptide from the amino-terminus of human Caspase-4.
• Buffer: Caspase-4 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: Caspase-4 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of caspase-4 in Ramos cells with caspase-4 antibody at (A) 0.5 and (B) 1 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemical staining of mouse spleen using caspase-4 antibody at 2 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of Caspase-4 in Mouse Spleen 100 cells with Caspase-4 antibody at μg/mL.)

Related Product Information for anti-CASP4 antibody

Caspase-4 Antibody: Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. Members of this subfamily include caspase-1, -4, -5, and -12. Activation of these caspases results in the cleavage and activation of proinflammatory cytokines such as IL-1β and IL-18. Caspase-4 was initially identified as a homologous protein to Caspase-1 and the C. elegans Ced-3 which could induce apoptosis in transfected cells. More recent studies have shown that it can be activated by ER stress and has been suggested to be involved in multiple neuronal pathologies such as Alzheimer's disease.

NCBI and Uniprot Product Information

• NCBI GI #: 886050
• NCBI GeneID: 837
• UniProt Accession #: P49662; A2NHL8; A2NHM0
• Molecular Weight: 36,705 Da
• NCBI Official Full Name: Ich-2
• NCBI Official Synonym Full Names: caspase 4, apoptosis-related cysteine peptidase
• NCBI Official Symbol: CASP4
• NCBI Official Synonym Symbols: TX; ICH-2; Mih1/TX; ICEREL-II; ICE(rel)II
• NCBI Protein Information: caspase-4; CASP-4; ICE(rel)-II; protease TX; protease ICH-2; apoptotic cysteine protease Mih1/TX; caspase 4, apoptosis-related cysteine protease
• UniProt Protein Name: Caspase-4
• Protein Family: Caspase
• UniProt Gene Name: CASP4
• UniProt Synonym Gene Names: ICH2; CASP-4
• UniProt Entry Name: CASP4_HUMAN

NCBI Description

This gene encodes a protein that is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain and a large and small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This caspase is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. When overexpressed, this gene induces cell apoptosis. Alternative splicing results in transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

CASP4: Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves caspase-1. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a small and a large subunit. Widely expressed, with highest levels in spleen and lung. Moderate expression in heart and liver, low expression in skeletal muscle, kidney and testis. Not found in the brain. Belongs to the peptidase C14A family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.22.64; Protease; Apoptosis

Chromosomal Location of Human Ortholog: 11q22.2-q22.3

Cellular Component: endoplasmic reticulum membrane; mitochondrion; cytoplasm

Molecular Function: cysteine-type endopeptidase activity

Biological Process: regulation of apoptosis; apoptosis; regulation of inflammatory response; proteolysis