Rabbit anti-Human BDKRB1 Polyclonal Antibody | anti-BDKRB1 antibody

BDKRB1, ID (B1 Bradykinin Receptor, BK-1 Receptor, B1R, BRADYB1) (MaxLight 490)

Cat. Num. AAA6465770

• Gene Names: BDKRB1; B1R; BKR1; B1BKR; BKB1R; BDKRB2; BRADYB1
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: BDKRB1; Polyclonal Antibody; ID (B1 Bradykinin Receptor; BK-1 Receptor; B1R; BRADYB1) (MaxLight 490); anti-BDKRB1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human BDKRB1.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.

• Applicable Applications for anti-BDKRB1 antibody: FLISA, Flow Cytometry (FC/FACS), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 213-239 in the central region of human BDKRB1.
• Conjugate: MaxLight490
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in HepG2 cell line lysates (35ug/lane) usingMBS629202. BDKRB1 (arrow) was detected usingMBS629202.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of HepG2 cells using MBS629202 (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-BDKRB1 antibody

Bradykinin, a 9aa peptide, is generated in pathophysiologic conditions such as inflammation, trauma, burns, shock, and allergy. Two types of G-protein coupled receptors have been found which bind bradykinin and mediate responses to these pathophysiologic conditions. BDKRB1 is one of these receptors and is synthesized de novo following tissue injury. Receptor binding leads to an increase in the cytosolic calcium ion concentration, ultimately resulting in chronic and acute inflammatory responses.

Product Categories/Family for anti-BDKRB1 antibody

Antibodies; Receptors

References

1. Bachvarov, D.R., et.al., Genomics 33(3), 374-381 (1996).

NCBI and Uniprot Product Information

• NCBI GI #: 20544172
• NCBI GeneID: 623
• NCBI Accession #: NP_000701.2
• NCBI GenBank Nucleotide #: NM_000710.3
• UniProt Accession #: P46663; Q546S7; Q8N0Y8; A8K7F5
• Molecular Weight: 40,495 Da
• NCBI Official Full Name: B1 bradykinin receptor
• NCBI Official Synonym Full Names: bradykinin receptor B1
• NCBI Official Symbol: BDKRB1
• NCBI Official Synonym Symbols: B1R; BKR1; B1BKR; BKB1R; BDKRB2; BRADYB1
• NCBI Protein Information: B1 bradykinin receptor; BK-1 receptor; bradykinin receptor 1; bradykinin B1 receptor; bradykinin receptor B2
• UniProt Protein Name: B1 bradykinin receptor
• Protein Family: B1 bradykinin receptor
• UniProt Gene Name: BDKRB1
• UniProt Synonym Gene Names: BRADYB1; B1R
• UniProt Entry Name: BKRB1_HUMAN

NCBI Description

Bradykinin, a 9 aa peptide, is generated in pathophysiologic conditions such as inflammation, trauma, burns, shock, and allergy. Two types of G-protein coupled receptors have been found which bind bradykinin and mediate responses to these pathophysiologic conditions. The protein encoded by this gene is one of these receptors and is synthesized de novo following tissue injury. Receptor binding leads to an increase in the cytosolic calcium ion concentration, ultimately resulting in chronic and acute inflammatory responses. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Uniprot Description

BKR1: This is a receptor for bradykinin. Could be a factor in chronic pain and inflammation. Belongs to the G-protein coupled receptor 1 family. Bradykinin receptor subfamily. BDKRB1 sub-subfamily.

Protein type: GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR

Chromosomal Location of Human Ortholog: 14q32.1-q32.2

Cellular Component: neuron projection; endoplasmic reticulum; integral to plasma membrane; plasma membrane

Molecular Function: bradykinin receptor activity; protein binding; peptide binding

Biological Process: cell migration; elevation of cytosolic calcium ion concentration; negative regulation of protein amino acid phosphorylation; response to mechanical stimulus; positive regulation of leukocyte migration; protein kinase C activation; response to lipopolysaccharide; negative regulation of cell growth; negative regulation of blood pressure; positive regulation of release of sequestered calcium ion into cytosol; sensory perception of pain; inflammatory response