SDS-Page
ECE1 recombinant protein
ECE1 Protein, Human, Recombinant (His Tag)
Gene Names
ECE1; ECE
Purity
>95% as determined by SDS-PAGE
Synonyms
ECE1; ECE1 Protein; Human; Recombinant (His Tag); Human ECE1 Protein (His Tag); ECE Protein; RP3-329E20.1 Protein; endothelin converting enzyme 1; ECE1 recombinant protein
Host
HEK293 Cells
Purity/Purification
>95% as determined by SDS-PAGE
Form/Format
Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Sequence
Gln90-Trp770
Species
Human
Endotoxin
<1.0EU per ug of the protein as determined by the LAL method
Predicted N Terminal
His
Tag
N-His
Protein Construction
A DNA sequence encoding the extracellular domain of human ECE1 isoform B (P42892-1)(Gln 90-Trp 770) was expressed, with a polyhistidine tag at the N-terminus.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
SDS-Page
SDS-Page
Related Product Information for ECE1 recombinant protein
Background: Endothelin-converting enzyme 1, also known as ECE-1, is a single-pass type II membrane protein which belongs to thepeptidase M13 family. ECE-1 converts big endothelin-1 to endothelin-1. ECE-1 is a membrane metalloprotease that generates endothelin from its direct precursor big endothelin. Four isoforms of ECE-1 are produced from a single gene through the use of alternate promoters. These isoforms share the same extracellular catalytic domain and contain unique cytosolic tails, which results in their specific subcellular targeting.All isoforms of ECE-1 are expressed in umbilical vein endothelial cells, polynuclear neutrophils, fibroblasts, atrium cardiomyocytes and ventricles. Isoforms A, B and C of ECE-1 are also expressed in placenta, lung, heart, adrenal gland and phaeochromocytoma; isoforms A and C of ECE-1 in liver, testis and small intestine; isoform B, C and D of ECE-1 in endothelial cells and umbilical vein smooth muscle cells; isoforms C and D in saphenous vein cells, and isoform C in kidney. Defects in ECE1 are a cause of Hirschsprung disease, cardiac defects and autonomic dysfunction. It is a form of Hirschsprung disease with skip-lesions defects, craniofacial abnormalities and other dysmorphic features, and autonomic dysfunction.
References
Yorimitsu K., et al.,(1995), Cloning and sequencing of a human endothelin converting enzyme in renal adenocarcinoma (ACHN) cells producing endothelin-2. Biochem. Biophys. Res. Commun. 208:721-727.Shimada K., et al., (1995), Cloning and functional expression of human endothelin-converting enzyme cDNA.Biochem. Biophys. Res. Commun. 207:807-812.Valdenaire O., et al.,(1995), Organization of the gene encoding the human endothelin-converting enzyme (ECE-1).J. Biol. Chem. 270:29794-29798.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
770
NCBI Official Full Name
endothelin-converting enzyme 1 isoform 3
NCBI Official Synonym Full Names
endothelin converting enzyme 1
NCBI Official Symbol
ECE1
NCBI Official Synonym Symbols
ECE
NCBI Protein Information
endothelin-converting enzyme 1; ECE-1
UniProt Protein Name
Endothelin-converting enzyme 1
Protein Family
UniProt Gene Name
ECE1
UniProt Synonym Gene Names
ECE-1
UniProt Entry Name
ECE1_HUMAN
Similar Products
Product Notes
The ECE1 ece1 (Catalog #AAA8120553) is a Recombinant Protein produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Gln90-Trp7 70. It is sometimes possible for the material contained within the vial of "ECE1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
