Rabbit Caspase-12 Polyclonal Antibody | anti-CASP12 antibody

Anti-Caspase-12 Antibody

Cat. Num. AAA4156952

• Gene Names: CASP12; Caspase-12
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Affinity Purified
• Synonyms: Caspase-12; Polyclonal Antibody; Anti-Caspase-12 Antibody; anti-CASP12 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This affinity purified antibody is directed against mouse Caspase-12 protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. The antibody is human, mouse and rat reactive. Reactivity against homologues from other sources has not been determined.
• Purity/Purification: Affinity Purified
• Form/Format: Liquid; PBS, pH 7.4 with 0.05% sodium azide.
• Concentration: 1ug/ul (varies by lot)
• Sequence Length: 485

• Applicable Applications for anti-CASP12 antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: This affinity purified antibody has been tested for use in western blotting and immunohistochemistry. Murine spleen tissue lysate can be used as a positive control in western blotting, and a band at approximately 53 kDa is expected.
• Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the amino terminus of mouse Caspase-12 protein.
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use.; For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Testing Data

Testing Data

Related Product Information for anti-CASP12 antibody

Three distinct signaling pathways lead to programmed cell death (apoptosis). Two of these are the death receptor and mitochondrion pathways, in which the key apoptotic proteases caspase-8 and caspase-9, respectively, are involved. Endoplasmic reticulum (ER) stress is the third apoptotic pathway and caspase-12 is involved (1, 2). Caspase-12 is localized to the ER but not to the cytoplasm or mitochondrion. Caspase-12 is activated by ER stress, including disruption of ER calcium homeostasis, and mediates ER stressinduced apoptosis. Caspase-12 is co-localized to the ER with several proteins that are involved in Alzheimer's disease, including gamma-secretase presenilin and beta-amyloid precursor protein (APP). Caspase-12 mediates cytotoxicity induced by amyloid-beta. Caspase-12 is ubiquitously expressed in mouse tissues (1).

NCBI and Uniprot Product Information

• NCBI GI #: 116175428
• NCBI GeneID: 479458
• NCBI GenBank Nucleotide #: NP_001070704.1
• UniProt Accession #: Q075B4
• NCBI Official Full Name: caspase-12
• NCBI Official Symbol: CASP12
• NCBI Official Synonym Symbols: Caspase-12
• NCBI Protein Information: caspase-12
• UniProt Protein Name: Caspase-12
• Protein Family: Caspase
• UniProt Gene Name: CASP-12
• UniProt Synonym Gene Names: CASP-12
• UniProt Entry Name: CASPC_CANFA

Uniprot Description

Function: Involved in the activation cascade of caspases responsible for apoptosis execution

By similarity.

Subunit structure: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by two subunits

Potential. May interact with TRAF2

By similarity.

Sequence similarities: Belongs to the peptidase C14A family.Contains 1 CARD domain.