Rabbit Caspase-12 Polyclonal Antibody | anti-Casp12 antibody

Caspase-12 Antibody (Small)

Cat. Num. AAA150848

• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Caspase-12 Antibody is (Small)affinity chromatography purified via peptide column.
• Synonyms: Caspase-12; Polyclonal Antibody; Caspase-12 Antibody (Small); Caspase-12 Antibody (Small): Caspase-12; CASP-12; caspase 12; anti-Casp12 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Caspase-12 Antibody is (Small)affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 419

• Applicable Applications for anti-Casp12 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Caspase-12 (small) antibody can be used for the detection of both the propeptide and cleaved forms of caspase-12 by Western blot at 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 10 mug/mL.
• Conjugate: Unconjugated
• Immunogen: caspase-12 antibody was raised against a synthetic peptide corresponding to amino acids within. The small cleavage product of murine caspase-12.
• Buffer: Caspase-12 Antibody is supplied in PBS containing 0.02% sodium azide. (Small)
• Preparation and Storage: Caspase-12 antibody (Small) can be stored at -20 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of caspase-12 in mouse (lane A) and rat (lane B) liver lysate with caspase-12 antibody (small) at 1 μg/mL. )

Immunohistochemistry (IHC)

(Immunohistochemical staining of human liver tissue using caspase-12 antibody (small) at 10 μg/mL.)

Related Product Information for anti-Casp12 antibody

Caspase-12 Antibody: (Small)Three distinct signaling pathways lead to programmed cell death (apoptosis). The death receptor and mitochondrion pathways are the main, in which the key apoptotic proteases capase-8 and caspase-9, respectively, are involved. The endoplasmic reticulum (ER) stress is the third apoptotic pathway and caspase-12 is involved. Caspase-12 is localized to the ER but not to cytoplasm or mitochondrion. Caspase-12 is activated by ER stress, including disruption of ER calcium homeostasis, and mediates ER stress-induced apoptosis. Caspase-12 is co-localized to the ER with several proteins that are involved in Alzheimer's disease including gamma-secretase presenilin and beta-amyloid precursor protein (APP). Caspase-12 mediates cytotoxicity induced by amyloid-beta. Caspase-12 is ubiquitously expressed in mouse tissues.

NCBI and Uniprot Product Information

• NCBI GI #: 2094806
• NCBI GeneID: 12364
• UniProt Accession #: O08736; Q3TT82
• Molecular Weight: 47,854 Da
• NCBI Official Full Name: caspase-12
• NCBI Official Synonym Full Names: caspase 12
• NCBI Official Symbol: Casp12
• NCBI Protein Information: caspase-12; CASP-12
• UniProt Protein Name: Caspase-12
• Protein Family: Caspase
• UniProt Gene Name: Casp12
• UniProt Synonym Gene Names: CASP-12
• UniProt Entry Name: CASPC_MOUSE

Uniprot Description

CASP12: Has no protease activity. May reduce cytokine release in response to bacterial lipopolysaccharide during infections. Reduces activation of NF-kappa-B in response to TNF. Detected in heart, kidney, liver, lung, pancreas, small intestine, spleen, stomach, thymus and testis. Belongs to the peptidase C14A family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.22.-; Protease

Cellular Component: intracellular membrane-bound organelle; endoplasmic reticulum; intracellular; cytosol; nucleus

Molecular Function: peptidase activity; protein binding; protease binding; hydrolase activity; endopeptidase activity; cysteine-type peptidase activity

Biological Process: regulation of apoptosis; protein autoprocessing; positive regulation of apoptosis; unfolded protein response; apoptosis; positive regulation of caspase activity; proteolysis