Mammalian Plasma Kallikrein Assay Kit

Plasma Kallikrein Inhibitor Screening Kit (Colorimetric)

Cat. Num. AAA846920

• Reactivity: Mammalian
• Synonyms: Plasma Kallikrein; Plasma Kallikrein Inhibitor Screening Kit (Colorimetric); Plasma Kallikrein assay kit

• Reactivity: Mammalian

• Application Notes: Screening/characterizing/studying Plasma Kallikrein inhibitors/activators
• Preparation and Storage: Store at -20 degree C for 12 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Plasma Kallikrein assay kit

Background/Introduction: Plasma Prekallikrein (EC 3.4.21.34), is the glycosylated single chain zymogen precursor of the plasma serine protease Kallikrein (PK). It circulates with kininogen and is activated by Factor XIIa to Kallikrein in the intrinsic coagulation pathway. Kallikrein activates plasminogen in fibrinolysis and cleaves kininogen in the bradykinin system of vasodilation. Prekallikrein deficiency is rare and causes increased activated partial thromboplastin time. Elevated plasma Prekallikrein is associated with diabetes and cardiovascular disease. Plasma Kallikrein inhibitors have been proposed as drugs to manage Hereditary Angioedema. The Plasma Kallikrein Inhibitor Screening Kit utilizes the ability of active Plasma Kallikrein to cleave a synthetic pNA-based peptide substrate to release pNA (OD405 nm), which can be easily quantified using a microplate reader. In the presence of a Plasma Kallikrein inhibitor, the cleavage of this substrate is reduced/abolished resulting in decrease or total loss of the pNA absorbance. This simple and high-throughput adaptable assay kit can be used to screen/study/characterize potential inhibitors of Plasma Kallikrein.

Product Categories/Family for Plasma Kallikrein assay kit

Proteases