Rabbit anti-Mouse, Rat Caspase-12 Polyclonal Antibody | anti-Casp12 antibody

Caspase-12 Antibody

Cat. Num. AAA841363

• Reactivity: Mouse, Rat
• Applications: Western Blot
• Purity: Affinity purified
• Synonyms: Caspase-12; Polyclonal Antibody; Caspase-12 Antibody; CASP12; CASP12P1; anti-Casp12 antibody

• Host: Rabbit
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purified
• Form/Format: 100 ug (0.2 mg/ml) affinity purified rabbit anti-caspase-12 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.; Appearance: Colorless liquid
• Concentration: 0.2 mg/ml (varies by lot)
• Sequence Length: 2605

• Applicable Applications for anti-Casp12 antibody: Western Blot (WB)
• Application Notes: Western blotting (0.5-4 ug/ml). It detects procaspase-12 (55 kDa) and cleavage caspase-12 (36 kDa) on SDS-PAGE immunoblots. However, the optimal conditions should be determined individually.
• Immunogen: Synthetic peptide corresponding to residues surrounding amino acid 310 of mouse caspase-12
• Handling: The antibody solution should be gently mixed before use.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for anti-Casp12 antibody

Background: Three distinct signaling pathways lead to programmed cell death (apoptosis). The death receptor and mitochondrion pathways are the mains two pathways, in which the key apoptotic proteases caspase-8 and caspase-9, respectively, are involved. The endoplasmic reticulum (ER) stress is the third apoptotic pathway and caspase-12 is involved. Caspase-12 is located to the ER, but not to the cytoplasm or mitochondrion. Caspase-12 is activated by ER stress, including disruption of ER calcium hemeostasis, and mediates ER stress-induced apoptosis. Caspase-12 is localized to the ER with several proteins that are involved in Alzheimer's disease including gamma-secretase presenilin and beta-amyloid precursor protein (APP). Caspase-12 mediates cytotoxicity induced by amyloid-beta. Caspase-12 is ubiquitously expressed in mouse tissues.

NCBI and Uniprot Product Information

• NCBI GI #: 157951683
• NCBI GeneID: 12364
• NCBI Accession #: NP_033938.3
• NCBI GenBank Nucleotide #: NM_009808
• Molecular Weight: 47,854 Da
• NCBI Official Full Name: Mus musculus caspase 12 (Casp12), mRNA
• NCBI Official Synonym Full Names: caspase 12
• NCBI Official Symbol: Casp12
• NCBI Protein Information: caspase-12
• UniProt Protein Name: Caspase-12
• Protein Family: Caspase
• UniProt Gene Name: Casp12
• UniProt Synonym Gene Names: CASP-12
• UniProt Entry Name: CASPC_MOUSE

Uniprot Description

CASP12: Has no protease activity. May reduce cytokine release in response to bacterial lipopolysaccharide during infections. Reduces activation of NF-kappa-B in response to TNF. Detected in heart, kidney, liver, lung, pancreas, small intestine, spleen, stomach, thymus and testis. Belongs to the peptidase C14A family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; EC 3.4.22.-

Cellular Component: intracellular membrane-bound organelle; endoplasmic reticulum; intracellular; nucleus; cytosol

Molecular Function: peptidase activity; protein binding; protease binding; hydrolase activity; endopeptidase activity; cysteine-type peptidase activity

Biological Process: regulation of apoptosis; protein autoprocessing; unfolded protein response; positive regulation of apoptosis; apoptosis; positive regulation of caspase activity; proteolysis