Western Blot (WB)
(Western Blot: Sample: Mouse Lung lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse CASP1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Mouse Caspase 1 (CASP1) Polyclonal Antibody | anti-CASP1 antibody
Polyclonal Antibody to Caspase 1 (CASP1)
MGHHHHHHSGSEF-NK EDGTFPGLTG TLKFCPLEKA QKLWKENPSE IYPIMNTTTR TRLALIICNT EFQHLSPRVG AQVDLREMKL LLEDLGYTVK VKENLTALEM VKEVKEFAAC PEHKTSDSTF LVFMSHGIQE GICGTTYSNE VSDILKVDTI FQMMNTLKCP SLKDKPKVII IQACRGEKQG VVLLKD
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Western Blot (WB)
(Western Blot: Sample: Mouse Lung lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse CASP1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Mouse Lung lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse CASP1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Mouse Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Mouse Tissue))
NCBI and Uniprot Product Information
Uniprot Description
CASP1: Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 10 kDa (p10) subunit. The p20 subunit can also form a heterodimer with the epsilon isoform which then has an inhibitory effect. May be a component of the inflammasome, a protein complex which also includes PYCARD, CARD8 and NALP2 and whose function would be the activation of proinflammatory caspases. Interacts with CARD17/INCA and CARD18. Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain. Specifically inhibited by the cowpox virus Crma protein. Belongs to the peptidase C14A family. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: Apoptosis; EC 3.4.22.36; Protease
Chromosomal Location of Human Ortholog: 9 A1|9 2.46 cM
Cellular Component: cytoplasm; extracellular region; IPAF inflammasome complex; neuron projection; NLRP1 inflammasome complex; NLRP3 inflammasome complex; nucleus; protein complex
Molecular Function: CARD domain binding; cysteine-type endopeptidase activity; cysteine-type peptidase activity; endopeptidase activity; hydrolase activity; identical protein binding; kinase binding; peptidase activity; protein binding
Biological Process: apoptosis; cellular response to interferon-gamma; cellular response to organic substance; interleukin-1 beta production; membrane hyperpolarization; memory; microglial cell activation; mitochondrial depolarization; myoblast fusion; positive regulation of apoptosis; positive regulation of circadian sleep/wake cycle, non-REM sleep; positive regulation of cysteine-type endopeptidase activity involved in apoptotic process; positive regulation of cytokine secretion; positive regulation of I-kappaB kinase/NF-kappaB signaling; positive regulation of interleukin-1 alpha secretion; positive regulation of interleukin-1 beta secretion; programmed necrotic cell death; protein autoprocessing; protein processing; proteolysis; pyroptosis; regulation of apoptosis; regulation of autophagy; regulation of inflammatory response; response to ATP; response to bacterium; response to drug; response to hypoxia; response to lipopolysaccharide; response to organic cyclic compound; toxin transport
Research Articles on CASP1
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Product Notes
The CASP1 casp1 (Catalog #AAA2006556) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Caspase 1 (CASP1) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Caspase 1 (CASP1) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the CASP1 casp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF-NK EDGTFPGLTG TLKFCPLEKA QKLWKENPSE IYPIMNTTTR TRLALIICNT EFQHLSPRVG AQVDLREMKL LLEDLGYTVK VKENLTALEM VKEVKEFAAC PEHKTSDSTF LVFMSHGIQE GICGTTYSNE VSDILKVDTI FQMMNTLKCP SLKDKPKVII IQACRGEKQG VVLLKD. It is sometimes possible for the material contained within the vial of "Caspase 1 (CASP1), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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