Mouse Caspase 1 (CASP1) ELISA Kit | CASP1 elisa kit

Mouse Caspase 1 (CASP1) ELISA Kit

Cat. Num. AAA455768

• Gene Names: Casp1; ICE; Il1bc
• Reactivity: Mouse
• Synonyms: Caspase 1 (CASP1); Mouse Caspase 1 (CASP1) ELISA Kit; CASP1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of CASP1. No significant cross-reactivity or interference between CASP1 and analogues was observed.
• Sequence Length: 402

• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.059ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CASP1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CASP1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Standard Curve/Testing Data

Related Product Information for CASP1 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CASP1 in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CASP1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to CASP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CASP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CASP1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 86198305
• NCBI GeneID: 12362
• NCBI Accession #: NP_033937.2
• NCBI GenBank Nucleotide #: NM_009807.2
• UniProt Accession #: P29452
• Molecular Weight: 45,640 Da
• NCBI Official Full Name: caspase-1
• NCBI Official Synonym Full Names: caspase 1
• NCBI Official Symbol: Casp1
• NCBI Official Synonym Symbols: ICE; Il1bc
• NCBI Protein Information: caspase-1
• UniProt Protein Name: Caspase-1
• UniProt Gene Name: Casp1
• UniProt Synonym Gene Names: Il1bc; CASP-1; IL-1BC; ICE; IL-1 beta-converting enzyme

Uniprot Description

Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis.