Testing Data
Rabbit anti-Human, Mouse Caspase 1 Polyclonal Antibody | anti-Casp1 antibody
Caspase 1 Antibody
30 ug (0.5 mg/ml) of antibody in PBS pH 7.2; 0.01 % BSA; 0.03 % ProClin; and 50 % glycerol.
The antibody solution should be gently mixed before use.
Testing Data
Testing Data
Caspase-1 (also known as ICE) is a prototypical member of the caspase-family of cysteine proteases. Caspase-1 exists in cells as an inactive 45 kDa proenzyme. The pro-enzyme is matured by proteolysis to yield large (20 kDa) and small (10 kDa) subunits. The active caspase-1 is a heterotetramer consisting of two large and two small subunits. To date the regulatory mechanism of caspase-1 activation and the role of caspase-1 in apoptosis are poorly understood. In THP-1 cells; a large proportion of the caspase-1 is present in the inactive proenzyme form.
NCBI and Uniprot Product Information
Uniprot Description
CASP1: Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 10 kDa (p10) subunit. The p20 subunit can also form a heterodimer with the epsilon isoform which then has an inhibitory effect. May be a component of the inflammasome, a protein complex which also includes PYCARD, CARD8 and NALP2 and whose function would be the activation of proinflammatory caspases. Interacts with CARD17/INCA and CARD18. Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain. Specifically inhibited by the cowpox virus Crma protein. Belongs to the peptidase C14A family. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: Apoptosis; EC 3.4.22.36; Protease
Chromosomal Location of Human Ortholog: 9 A1|9 2.46 cM
Cellular Component: cytoplasm; extracellular region; neuron projection; nucleus; protein complex
Molecular Function: cysteine-type endopeptidase activity; endopeptidase activity; identical protein binding; kinase binding; peptidase activity; protein binding
Biological Process: interleukin-1 beta production; membrane hyperpolarization; memory; microglial cell activation; mitochondrial depolarization; myoblast fusion; positive regulation of apoptosis; positive regulation of circadian sleep/wake cycle, non-REM sleep; positive regulation of cytokine secretion; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-1 alpha secretion; positive regulation of interleukin-1 beta secretion; protein processing; proteolysis; regulation of autophagy; regulation of inflammatory response; response to ATP; response to bacterium; response to drug; response to hypoxia; response to lipopolysaccharide; response to organic cyclic substance
Research Articles on Casp1
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Product Notes
The Casp1 casp1 (Catalog #AAA845520) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Caspase 1 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Caspase 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western Blot: 1-4 ug. Researchers should empirically determine the suitability of the Casp1 casp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Caspase 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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