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Western Blot (WB) (Fig.1. Western Blot analysis of various cells using CD31 Polyclonal Antibody diluted at 1:1000.)

Rabbit anti-Human, Mouse CD31 Polyclonal Antibody | anti-CD31 antibody

CD31 Polyclonal Antibody

Gene Names
PECAM1; CD31; PECA1; GPIIA'; PECAM-1; endoCAM; CD31/EndoCAM
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
CD31; Polyclonal Antibody; CD31 Polyclonal Antibody; Rabbit Anti-CD31 Polyclonal Antibody; PECAM1; Platelet endothelial cell adhesion molecule; PECAM-1; EndoCAM; GPIIA'; PECA1; CD antigen CD31; anti-CD31 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
CD31 Polyclonal Antibody detects endogenous levels of CD31 protein.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Liquid; PBS Containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Concentration
1 mg/ml (varies by lot)
Sequence Length
738
Applicable Applications for anti-CD31 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC-P: 1:100-1:300
IF: 1:200-1:1000
ELISA: 1:20000
Immunogen
Synthesized peptide derived from human CD31 around the non-phosphorylation site of Y713
Preparation and Storage
Stable for one year at -20 degree C from date of shipment.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Western Blot (WB)

(Fig.1. Western Blot analysis of various cells using CD31 Polyclonal Antibody diluted at 1:1000.)

Western Blot (WB) (Fig.1. Western Blot analysis of various cells using CD31 Polyclonal Antibody diluted at 1:1000.)

Western Blot (WB)

(Fig.2. Western Blot analysis of rat-musle cells using CD31 Polyclonal Antibody diluted at 1:1000.)

Western Blot (WB) (Fig.2. Western Blot analysis of rat-musle cells using CD31 Polyclonal Antibody diluted at 1:1000.)

Western Blot (WB)

(Fig.3. Western blot analysis of KB lysis using CD31 antibody. Antibody was diluted at 1:1000.)

Western Blot (WB) (Fig.3. Western blot analysis of KB lysis using CD31 antibody. Antibody was diluted at 1:1000.)

Western Blot (WB)

(Fig.4. Western blot analysis of 293T lysis using CD31 antibody. Antibody was diluted at 1:1000.)

Western Blot (WB) (Fig.4. Western blot analysis of 293T lysis using CD31 antibody. Antibody was diluted at 1:1000.)
Related Product Information for anti-CD31 antibody
Platelet and endothelial cell adhesion molecule 1 encoded by PECAM1 is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
platelet endothelial cell adhesion molecule
NCBI Official Synonym Full Names
platelet and endothelial cell adhesion molecule 1
NCBI Official Symbol
PECAM1
NCBI Official Synonym Symbols
CD31; PECA1; GPIIA'; PECAM-1; endoCAM; CD31/EndoCAM
NCBI Protein Information
platelet endothelial cell adhesion molecule
UniProt Protein Name
Platelet endothelial cell adhesion molecule
UniProt Gene Name
PECAM1
UniProt Synonym Gene Names
PECAM-1
UniProt Entry Name
PECA1_HUMAN

NCBI Description

The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [provided by RefSeq, May 2010]

Uniprot Description

PECAM-1: a cell adhesion molecule expressed on platelets and at endothelial cell intercellular junctions. Plays a role in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, cell migration, negative regulation of immune cell signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. Undergoes alternative splicing, generating multiple isoforms in vascular beds of various tissues.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 17q23.3

Cellular Component: extracellular space; platelet alpha granule membrane; integral to membrane; plasma membrane; cell junction

Molecular Function: protein binding

Biological Process: platelet activation; extracellular matrix organization and biogenesis; platelet degranulation; diapedesis; cell recognition; blood coagulation; signal transduction; cell adhesion; leukocyte migration; phagocytosis

Research Articles on CD31

Similar Products

Product Notes

The CD31 pecam1 (Catalog #AAA9701633) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CD31 Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD31 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), ELISA (EIA). WB: 1:500-1:2000 IHC-P: 1:100-1:300 IF: 1:200-1:1000 ELISA: 1:20000. Researchers should empirically determine the suitability of the CD31 pecam1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD31, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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