Rabbit anti-Human BCL2A1 Polyclonal Antibody | anti-BCL2A1 antibody

BCL2A1, BH3 Domain Specific (BCL2A1, BCL2L5, BFL1, GRS, HBPA1, Bcl-2-related protein A1, Bcl-2-like protein 5, Hemopoietic-specific early response protein, Protein BFL-1, Protein GRS) (AP)

Cat. Num. AAA6277955

• Gene Names: BCL2A1; GRS; BFL1; ACC-1; ACC-2; HBPA1; BCL2L5
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: BCL2A1; Polyclonal Antibody; BH3 Domain Specific (BCL2A1; BCL2L5; BFL1; GRS; HBPA1; Bcl-2-related protein A1; Bcl-2-like protein 5; Hemopoietic-specific early response protein; Protein BFL-1; Protein GRS) (AP); Protein GRS; anti-BCL2A1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human BCL2A1, BH3 Domain.
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-BCL2A1 antibody: Immunohistochemistry (IHC) Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 30-65 of human BCL2A1, BH3 Domain.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in human placenta tissue lysate usingMBS6007956. Bcl2-related Protein A1 (arrow) was detected usingMBS6007956. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human cancer tissue reacted withMBS6007956, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS6007956 for immunohistochemistry. BC = breast carcinoma.)

Product Categories/Family for anti-BCL2A1 antibody

Antibodies; Apoptosis

References

1. Akatsuka, Y., et al., J. Exp. Med. 197(11):1489-1500 (2003). 2. Edelstein, L.C., et al., Mol. Cell. Biol. 23(8):2749-2761 (2003). 3. Werner, A.B., et al., J. Biol. Chem. 77(25):22781-22788 (2002). 4. Akari, H., et al., J. Exp. Med. 194(9):1299-1311 (2001). 5. Harrington, J.J., et al., Nat. Biotechnol. 19(5):440-445 (2001).

NCBI and Uniprot Product Information

• NCBI GI #: 2493280
• NCBI GeneID: 597
• UniProt Accession #: Q16548; Q6FGZ4; Q6FH19; Q86W13; Q99524
• Molecular Weight: 175
• NCBI Official Full Name: Bcl-2-related protein A1
• NCBI Official Synonym Full Names: BCL2-related protein A1
• NCBI Official Symbol: BCL2A1
• NCBI Official Synonym Symbols: GRS; BFL1; ACC-1; ACC-2; HBPA1; BCL2L5
• NCBI Protein Information: bcl-2-related protein A1; bcl2-L-5; protein BFL-1; bcl-2-like protein 5; hematopoietic BCL2-related protein A1; hemopoietic-specific early response protein
• UniProt Protein Name: Bcl-2-related protein A1
• Protein Family: Bcl-2-related protein
• UniProt Gene Name: BCL2A1
• UniProt Synonym Gene Names: BCL2L5; BFL1; GRS; HBPA1; Bcl2-L-5
• UniProt Entry Name: B2LA1_HUMAN

NCBI Description

This gene encodes a member of the BCL-2 protein family. The proteins of this family form hetero- or homodimers and act as anti- and pro-apoptotic regulators that are involved in a wide variety of cellular activities such as embryonic development, homeostasis and tumorigenesis. The protein encoded by this gene is able to reduce the release of pro-apoptotic cytochrome c from mitochondria and block caspase activation. This gene is a direct transcription target of NF-kappa B in response to inflammatory mediators, and is up-regulated by different extracellular signals, such as granulocyte-macrophage colony-stimulating factor (GM-CSF), CD40, phorbol ester and inflammatory cytokine TNF and IL-1, which suggests a cytoprotective function that is essential for lymphocyte activation as well as cell survival. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

BCL2A1: Retards apoptosis induced by IL-3 deprivation. May function in the response of hemopoietic cells to external signals and in maintaining endothelial survival during infection. Interacts directly with BAK1, BID, BMF and BBC3. Interacts directly with BCL2L11/BIM. Interacts with BAX isoform Sigma. Interacts directly with PMAIP1. By phorbol ester and inflammatory cytokines, such as TNF or IL1B/interleukin-1 beta, but not by growth factors. Seems to be restricted to the hematopoietic compartment. Expressed in peripheral blood, spleen, and bone marrow, at moderate levels in lung, small intestine and testis, at a minimal levels in other tissues. Also found in vascular smooth muscle cells and hematopoietic malignancies. Belongs to the Bcl-2 family. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 15q24.3

Cellular Component: mitochondrial outer membrane

Molecular Function: BH domain binding; protein binding; protein homodimerization activity; protein heterodimerization activity

Biological Process: DNA damage response, signal transduction resulting in induction of apoptosis; negative regulation of apoptosis