Rabbit anti-Human, Mouse STAT4 Polyclonal Antibody | anti-STAT4 antibody

STAT4 antibody (pAb)

Cat. Num. AAA388928

• Gene Names: STAT4; SLEB11
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Affinity Purified
• Synonyms: STAT4; Polyclonal Antibody; STAT4 antibody (pAb); SLEB11; anti-STAT4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purified
• Form/Format: Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

• Applicable Applications for anti-STAT4 antibody: Western Blot (WB)
• Application Notes: Applications Validated:; WB: 1:500-1:2,000 dilution; The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot.
• Immunogen: This antibody was raised against a peptide within the C-terminal region of human STAT4.
• Preparation and Storage: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul

Western Blot (WB)

(STAT4 antibody (pAb) tested by Western blot. Nuclear extract of mouse testis (30 ug) probed with STAT4 antibody (pAb) at a dilution of 1:2,000.)

Related Product Information for anti-STAT4 antibody

Short Description: Our STAT4 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Western blot; it has been shown to react with Human and Mouse samples.

Background: STAT4 (Signal Transducer And Activator Of Transcription 4) is involved in gene activation in response to IL-12, and is required for the development of the Th1 cell lineage. Both IL-12 and IFNa are capable of activating STAT4. Variants of the STAT4 gene are frequently associated with numerous auto-immune disorders.

Product Categories/Family for anti-STAT4 antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 4507255
• NCBI GeneID: 6775
• NCBI Accession #: NP_003142; AB_2793663
• NCBI GenBank Nucleotide #: NM_003151
• UniProt Accession #: Q14765
• Molecular Weight: 86kDa
• NCBI Official Full Name: signal transducer and activator of transcription 4
• NCBI Official Synonym Full Names: signal transducer and activator of transcription 4
• NCBI Official Symbol: STAT4
• NCBI Official Synonym Symbols: SLEB11
• NCBI Protein Information: signal transducer and activator of transcription 4
• UniProt Protein Name: Signal transducer and activator of transcription 4
• Protein Family: Signal transducer and activator of transcription
• UniProt Gene Name: STAT4
• UniProt Entry Name: STAT4_HUMAN

NCBI Description

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is essential for mediating responses to IL12 in lymphocytes, and regulating the differentiation of T helper cells. Mutations in this gene may be associated with systemic lupus erythematosus and rheumatoid arthritis. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Aug 2011]

Uniprot Description

STAT4: transcription factor of the STAT family. Phosphorylated and activated by receptor-associated kinases downstream of many cytokines and growth-factor receptors. Essential for mediating responses to IL12 in lymphocytes, and regulating the differentiation of T helper cells.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 2q32.2-q32.3

Cellular Component: nucleoplasm; cytoplasm

Molecular Function: signal transducer activity; protein binding; sequence-specific DNA binding; transcription factor activity

Biological Process: cell proliferation; transcription, DNA-dependent; cytokine and chemokine mediated signaling pathway; positive regulation of transcription from RNA polymerase II promoter; JAK-STAT cascade; protein amino acid phosphorylation

Disease: Systemic Lupus Erythematosus, Susceptibility To, 11